4.2.2.1: hyaluronate lyase

This is an abbreviated version!
For detailed information about hyaluronate lyase, go to the full flat file.

Word Map on EC 4.2.2.1

4.2.2.1

streptococcus

chondroitin

disaccharide

glycosaminoglycans

agalactiae

chondroitinase

enteroaggregative

hyaluronidases

medicine

hyals

hyalurolyticus

pharmacology

autotransporter

degradation

synthesis

analysis

Reaction

(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1

+ 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine +

Synonyms

acidic hyase, bacterial HAse, bacterial hyaluronidase, bacterial spreading factor, bacteriophage-associated hyaluronate lyase, Cumulase, EC 4.2.99.1, GBS HA lyase, GBS hyase, glucuronoglycosaminoglycan lyase, h-SD, h-SH, HA lyase, HL, Hyal, Hyal-1, Hyal-2, HYAL1, Hyal2, HYAL3, hyaluronan lyase, hyaluronate lyase, hyaluronate lyase HylA, hyaluronate lyase HylP2, hyaluronidase, hyaluronidase 3, hyaluronidase SD, hyaluronidase SH, hyaluronidase-1, hyaluronidase-2, hyaluronoglucosaminidase-1, hyaluronoglucosaminidase-2, HYase, HylA7, hylB, hylB4755, HylP, Hylp2, LUCA-1, LUCA-2, lyase, glucuronoglycosaminoglycan, lyase, hyaluronate, More, mucinase, protein SEQ2045, rHuPH20, SagHL, SagHyal, spnHL, SpnHyal, spreading factor, SP_0314

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.2 Acting on polysaccharides

4.2.2.1 hyaluronate lyase

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Results	in table
5	Activating Compound
415	AA Sequence
16	Application
1	CAS Registry Number
42	Cloned(Commentary)
17	Crystallization (Commentary)
119	Disease/ Diagnostics
1	Expression
18	General Information
4	General Stability
45	IC50 Value
153	Inhibitors
2	Ki Value [mM]
24	KM Value [mM]
26	Localization
17	Metals/Ions
44	Molecular Weight [Da]
66	Natural Substrates/ Products (Substrates)
221	Organism
1	Oxidation Stability
1	Pathway
48	PDB ID
34	pH Optimum
8	pH Range
13	pH Stability
1	pI Value
8	Posttranslational Modification
91	Protein Variants
28	Purification (Commentary)
63	Reaction
6	Reaction Type
160	Reference
16	Source Tissue
20	Specific Activity [micromol/min/mg]
5	Storage Stability
206	Substrates and Products (Substrate)
44	Subunits
147	Synonyms
1	Systematic Name
23	Temperature Optimum [°C]
4	Temperature Range [°C]
19	Temperature Stability [°C]
8	Turnover Number [1/s]

Purification

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Purification on EC 4.2.2.1 - hyaluronate lyase

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9 entries

cloning overexpression, and purification of SagHL and its different fragments/domains

Streptococcus agalactiae

Q53591

680688

cloning, overexpression and purification of the C-terminal domain swapped chimeras of SpnHL and SagHL, modified enzyme SpnHLv/SagHLv (where the size of linker connecting the alpha-domain to C-terminal domain is enlarged by two amino acid)

Streptococcus pneumoniae

from a culture broth

isolated N-terminal domain from purified recombinant native enzyme

Streptococcus pneumoniae

Q54873

656140

native enzyme from serotype 7 to over 95% purity, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)

Streptococcus suis

Q8VLQ8

666251

partial, isoenzyme A, B and C

Staphylococcus aureus

210738

partially, recombinant enzyme from Escherichia coli

Streptococcus pyogenes phage H4489A

654497

purification from the supernatant by ammonium sulfate fractionation, Q Sepharose Fast Flow, and Sephadex G-100 chromatography

Arthrobacter globiformis

746795

recombinant enzyme

2 entries

recombinant enzyme from Escherichia coli

Streptococcus agalactiae

Q53591

656077

recombinant His-tagged enzyme 53.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cation exchange chromatography

Streptococcus pyogenes phage H4489A

714651

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chomatography, dialysis, gel filtration and ultrafiltration

Streptococcus suis

728911

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by ion exchange and nickel affinity chromatography, and gel filtration

Streptococcus pyogenes phage H10403

665742

recombinant wild-type and mutant enzymes from Escherichia coli

3 entries

type 24, strain G54

Streptococcus pyogenes

210741