Cloned (Comment) | Organism |
---|---|
overexpression of the enzymes from strains 3502 and 4755 in Escherichia coli | Streptococcus agalactiae |
Crystallization (Comment) | Organism |
---|---|
enzyme-hyaluronate hexasaccharide substrate complex, hanging drop vapour diffusion method, room temperature, in 10-50 mM hyaluronate hexasaccharide, 0.1 M sodium cacodylate, pH 6.0, 30 mM potassium thiocyanate, 30% PEG monomethyl ester 5000, several days, X-ray diffraction structure determination and analysis at 2.2 A resolution | Streptococcus agalactiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92000 | - |
x * 92000, SDS-PAGE | Streptococcus agalactiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronan | Streptococcus agalactiae | elimination of the polymer in the host connective tissue | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus agalactiae | Q53591 | strains 3502, gene hyl3502, and 4755, gene hyl4755 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Streptococcus agalactiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate | catalytic mechanism, the flexibility of the enzyme structure is important, the beta-domain is involved in the progressive catalytic process, enzyme substrate interactions | Streptococcus agalactiae |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronan | - |
Streptococcus agalactiae | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine | unsaturated disaccharide | ? | |
hyaluronan | elimination of the polymer in the host connective tissue | Streptococcus agalactiae | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine | - |
? | |
hyaluronate hexasaccharide | - |
Streptococcus agalactiae | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine | unsaturated disaccharide | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 92000, SDS-PAGE | Streptococcus agalactiae |
More | enzyme is composed of 2 domains, alpha-helical alpha-domain and beta-sheet beta-domain, which are structurally and functionally distinct | Streptococcus agalactiae |