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Literature summary for 4.2.2.1 extracted from
- Staphylococcal hyaluronate lyase (1973), Contrib. Microbiol. Immunol., 1, 376-389.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Staphylococcus aureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Staphylococcus aureus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
isoenzyme C, gel filtration | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | contains neutral sugars galactose, glucose, and mannose in the ratio 1:3:6. Amino sugars galactosamine and glucosamine, or mannosamine are present in the ratio 1:1 | Staphylococcus aureus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, isoenzyme A, B and C | Staphylococcus aureus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 1 year | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronate | - |
Staphylococcus aureus | alpha-4-deoxy-L-threo-hex-4-enopyranosyluronic acid-beta-1,3--N-acetyl-glucosamine | - |
? |  |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Staphylococcus aureus |
| 20 | - |
room temperaure, stable | Staphylococcus aureus |
| 37 | - |
stable | Staphylococcus aureus |
| 37 | - |
1 h, stable | Staphylococcus aureus |
| 56 | - |
unstable at | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
and a second optimum at pH 8.6 | Staphylococcus aureus |
| 8.6 | - |
and a second optimum at pH 6.0 | Staphylococcus aureus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | - |
18 h, complete loss of activity | Staphylococcus aureus |
| 2 | 13 | 18 h, stable | Staphylococcus aureus |
| 14 | - |
18 h, complete loss of activity | Staphylococcus aureus |
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Release 2023.1 (January 2023)
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