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Literature summary for 4.2.2.1 extracted from

  • Akhtar, M.S.; Bhakuni, V.
    Streptococcus pneumoniae hyaluronate lyase: an overview (2004), Curr. Sci., 86, 285-295.
No PubMed abstract available
Search for more literature for 4.2.2.1

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is synthesized as inactive zymogen in the cell and then secreted and activated by cleavage of the signal peptide Streptococcus pneumoniae
additional information the enzyme is synthesized as inactive zymogen in the cell and then secreted and activated by cleavage of the signal peptide Streptococcus agalactiae

Application

Application Comment Organism
medicine the enzyme can be used as a vaccine Streptococcus pneumoniae
pharmacology the enzyme is a target for structure-based design of selective inhibitors as drugs in bacterial infection therapy Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli resulting in different protein sizes Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
H399A the mutant enzyme shows 12% of wild-type enzyme activity Streptococcus pneumoniae
additional information mutation of the catalytic residues Asn429, His479, and Tyr488 inactivates the enzyme Streptococcus agalactiae
N349A the mutant enzyme shows 6% of wild-type enzyme activity Streptococcus pneumoniae
N580G the mutant enzyme shows 115% of wild-type enzyme activity Streptococcus pneumoniae
R243V the mutant enzyme shows 67% of wild-type enzyme activity Streptococcus pneumoniae
Y408F the mutant enzyme is inactive Streptococcus pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Streptococcus pneumoniae 9986
-
cell surface
-
 Streptococcus agalactiae 9986
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91000 107000 the recombinantly expressed enzyme exists in different proteolytically fragmented forms of the full-length enzyme with Mr of 107 kDa Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hyaluronan Streptococcus pneumoniae
-
 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine disaccharide unit ir
hyaluronan Streptococcus agalactiae
-
 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine disaccharide unit ir
additional information Streptococcus pneumoniae the enzyme is involved in degradation of hyaluronate from connective host tissues to facilitate the host and specific tissue invasion by the bacterium, overview ?
-
 ?
additional information Streptococcus agalactiae the enzyme is involved in degradation of hyaluronate from connective host tissues to facilitate the host and specific tissue invasion by the bacterium, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus agalactiae
-
-
-
Streptococcus pneumoniae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme is synthesized as inactive zymogen in the cell and then secreted and activated by cleavage of the signal peptide Streptococcus agalactiae
proteolytic modification the enzyme is synthesized as inactive zymogen int he cell and then secreted and activated by cleavage of the signal peptide Streptococcus pneumoniae

Reaction

Reaction Comment Organism Reaction ID
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate catalytic reaction mechanism and structural requirements, catalytic residues are Asn349, His399, and Tyr408 Streptococcus pneumoniae
a
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate catalytic reaction mechanism and structural requirements, catalytic residues are Asn429, His479, and Tyr488, a required hydrophobic patch is composed of residues Glu468, Asp478, and Thr480 Streptococcus agalactiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronan
-
 Streptococcus pneumoniae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine disaccharide unit ir
hyaluronan
-
 Streptococcus agalactiae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine disaccharide unit ir
hyaluronan substrate structure Streptococcus pneumoniae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine disaccharide unit ir
hyaluronan substrate structure Streptococcus agalactiae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine disaccharide unit ir
additional information the enzyme is involved in degradation of hyaluronate from connective host tissues to facilitate the host and specific tissue invasion by the bacterium, overview Streptococcus pneumoniae ?
-
 ?
additional information the enzyme is involved in degradation of hyaluronate from connective host tissues to facilitate the host and specific tissue invasion by the bacterium, overview Streptococcus agalactiae ?
-
 ?

Subunits

Subunits Comment Organism
More the three-dimensional enzyme structure contains a catalytic alpha-domain at the N-terminus and a C-terminal supportive cell-wall-anchoring beta-domain connected by a short peptide linker Streptococcus pneumoniae
More the three-dimensional enzyme structure contains a catalytic alpha-domain at the N-terminus and a C-terminal supportive cell-wall-anchoring beta-domain connected by a short peptide linker Streptococcus agalactiae

Synonyms

Synonyms Comment Organism
HL
-
 Streptococcus pneumoniae
HL
-
 Streptococcus agalactiae