4.2.1.9: dihydroxy-acid dehydratase

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For detailed information about dihydroxy-acid dehydratase, go to the full flat file.

Reaction

Synonyms

2,3-dihydroxy acid hydrolyase, 2,3-dihydroxyisovalerate dehydratase, acetohydroxyacid dehydratase, AfIlv3A, AFUA_2G2410, alpha,beta-dihydroxy acid dehydratase, alpha,beta-dihydroxyisovalerate dehydratase, AT3G23940, DAD, dehydratase, dihydroxy acid, DHAD, dihydroxy acid dehydrase, dihydroxy acid dehydratase, dihydroxyacid dehydratase, H16_A2987, ilvC, IlvD, SsDHAD, VEG110, Vegetative protein 110

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.9 dihydroxy-acid dehydratase

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Results	in table
2	Activating Compound
49691	AA Sequence
5	Application
1	CAS Registry Number
10	Cloned(Commentary)
2	Cofactor
3	Expression
14	General Information
1	General Stability
6	IC50 Value
55	Inhibitors
1	Ki Value [mM]
27	KM Value [mM]
5	Localization
26	Metals/Ions
10	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
260	Organism
8	Oxidation Stability
14	Pathway
16	PDB ID
18	pH Optimum
4	pH Range
6	Protein Variants
10	Purification (Commentary)
3	Reaction
1	Reaction Type
60	Reference
10	Source Tissue
16	Specific Activity [micromol/min/mg]
3	Storage Stability
141	Substrates and Products (Substrate)
6	Subunits
48	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
10	Temperature Stability [°C]
14	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 4.2.1.9 - dihydroxy-acid dehydratase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, about 85% loss of activity after 4 weeks, crude extract is unstable upon storage

Escherichia coli

-

33984

-80°C, after purification of His-tagged enzyme the total enzyme activity is decreased to 43% of the crude extract and even further to 17% when stored at -80 °C for one day

Saccharolobus solfataricus

Q97UB2

732214

-80°C, specific activity for D-gluconate of the frozen/thawed enzyme is stable for 60 min, but loses about 70% after a maximum of 100 min

Saccharolobus solfataricus

Q97UB2

732214