4.2.1.9: dihydroxy-acid dehydratase
This is an abbreviated version!
For detailed information about dihydroxy-acid dehydratase, go to the full flat file.
Word Map on EC 4.2.1.9
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4.2.1.9
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branched-chain
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dhads
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fe-s
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acetolactate
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isomeroreductase
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isobutanol
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2-ketoisovalerate
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reductoisomerase
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2,3-butanediol
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bcaas
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ketol-acid
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ilvbncd
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drug development
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analysis
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synthesis
- 4.2.1.9
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branched-chain
-
dhads
- fe-s
- acetolactate
-
isomeroreductase
- isobutanol
- 2-ketoisovalerate
- reductoisomerase
- 2,3-butanediol
-
bcaas
-
ketol-acid
-
ilvbncd
- drug development
- analysis
- synthesis
Reaction
Synonyms
2,3-dihydroxy acid hydrolyase, 2,3-dihydroxyisovalerate dehydratase, acetohydroxyacid dehydratase, AfIlv3A, AFUA_2G2410, alpha,beta-dihydroxy acid dehydratase, alpha,beta-dihydroxyisovalerate dehydratase, AT3G23940, DAD, dehydratase, dihydroxy acid, DHAD, dihydroxy acid dehydrase, dihydroxy acid dehydratase, dihydroxyacid dehydratase, H16_A2987, ilvC, IlvD, SsDHAD, VEG110, Vegetative protein 110
ECTree
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Engineering
Engineering on EC 4.2.1.9 - dihydroxy-acid dehydratase
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additional information
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construction of a valine producer by genetic engineering, mutant strains are resistant against inhibition by branched-chain amino acids L-valine, L-isoleucine and L-leucine, A constructed weak promoter of ilvA or leuA, which is introduced into the Corynebacterium glutamicum chromosome via a gene-replacement technique, reduces the biosynthetic rate of isoleucine or leucine, which lowers the mutant growth rate and increases valine production. Overexpression of ilvD driven by the strong mutant promoter P-ilvDM7 resultsin an even higher level of valine production, engineered strains, overview
additional information
construction of a relaxed rel deletion Corynebacterium glutamicum DELTAilvA DELTApanB DELTArel ilvNM13 (pECKAilvBNC) strain with the ilvBNC operon present on the multicopy plasmid pECKA
additional information
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construction of a relaxed rel deletion Corynebacterium glutamicum DELTAilvA DELTApanB DELTArel ilvNM13 (pECKAilvBNC) strain with the ilvBNC operon present on the multicopy plasmid pECKA
additional information
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engineering of wild-type Corynebacterium glutamicum for growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production is further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the constructed strains produce up to 188 mM 2-ketoisovalerate and show a product yield of about 0.47 mol per mol of glucose and a volumetric productivity of about 4.6 mM 2-ketoisovalerate per h in the overall production phase, overview
additional information
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construction of a relaxed rel deletion Corynebacterium glutamicum DELTAilvA DELTApanB DELTArel ilvNM13 (pECKAilvBNC) strain with the ilvBNC operon present on the multicopy plasmid pECKA
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additional information
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construction of strain BL21(DE3) DELTAilvD knockout mutant, functional complementation by Mycobacterium tuberculosis IlvD
additional information
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enzyme from iv-1 mutant strain 332 differs from the wild type enzyme with respect to heat-inactivation, pH-dependence, and kinetics. The altered structure of the mutant enzyme is the basic defect hindering its incorporation into mitochondria