4.2.1.9: dihydroxy-acid dehydratase

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For detailed information about dihydroxy-acid dehydratase, go to the full flat file.

Reaction

Synonyms

2,3-dihydroxy acid hydrolyase, 2,3-dihydroxyisovalerate dehydratase, acetohydroxyacid dehydratase, AfIlv3A, AFUA_2G2410, alpha,beta-dihydroxy acid dehydratase, alpha,beta-dihydroxyisovalerate dehydratase, AT3G23940, DAD, dehydratase, dihydroxy acid, DHAD, dihydroxy acid dehydrase, dihydroxy acid dehydratase, dihydroxyacid dehydratase, H16_A2987, ilvC, IlvD, SsDHAD, VEG110, Vegetative protein 110

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.9 dihydroxy-acid dehydratase

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Results	in table
2	Activating Compound
49691	AA Sequence
5	Application
1	CAS Registry Number
10	Cloned(Commentary)
2	Cofactor
3	Expression
14	General Information
1	General Stability
6	IC50 Value
55	Inhibitors
1	Ki Value [mM]
27	KM Value [mM]
5	Localization
26	Metals/Ions
10	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
260	Organism
8	Oxidation Stability
14	Pathway
16	PDB ID
18	pH Optimum
4	pH Range
6	Protein Variants
10	Purification (Commentary)
3	Reaction
1	Reaction Type
60	Reference
10	Source Tissue
16	Specific Activity [micromol/min/mg]
3	Storage Stability
141	Substrates and Products (Substrate)
6	Subunits
48	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
10	Temperature Stability [°C]
14	Turnover Number [1/s]

Oxidation Stability

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Oxidation Stability on EC 4.2.1.9 - dihydroxy-acid dehydratase

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OXIDATION STABILITY

ORGANISM

UNIPROT

LITERATURE

5% O2, at 25°C, half-life: less than 6 min, under anaerobic conditions the enzyme is stable for more than 8 days

Methanococcus aeolicus

-

33983

exposure of E. coli cells to 4.2 atm O2 causes complete loss of activity

Escherichia coli

-

33973

hyperbaric oxygen results in rapid inactivation

Escherichia coli

-

33971, 33975

inactivation by exposure to hyperbaric O2 is due to the destruction of its catalytically active 4Fe-4S cluster. Reactivation occurs by reconstitution of the enzyme's Fe-S cluster

Escherichia coli

-

33977

oxidative degradation appears to lead to a complete breakdown of the Fe-S cluster, and the resulting protein cannot by reactivated with Fe2+ and thiol reducing agents

Escherichia coli

-

33976

when the recombinant enzyme is incubated in 50 mM Tris-HCl buffer at 30°C for 2 h under aerobic or anaerobic conditions and the residual activity is measured, no difference is found between aerobic and anaerobic conditions

Saccharolobus solfataricus

Q97UB2

680539

when the recombinant enzyme is incubated in 50 mM Tris-HCl buffer at 30°C for 2 h with organic peroxide, the enzyme activity is stable during exposure to 0.5% organic peroxide for 2 h

Saccharolobus solfataricus

Q97UB2

680539