4.2.1.9: dihydroxy-acid dehydratase
This is an abbreviated version!
For detailed information about dihydroxy-acid dehydratase, go to the full flat file.
Word Map on EC 4.2.1.9
-
4.2.1.9
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branched-chain
-
dhads
-
fe-s
-
acetolactate
-
isomeroreductase
-
isobutanol
-
2-ketoisovalerate
-
reductoisomerase
-
2,3-butanediol
-
bcaas
-
ketol-acid
-
ilvbncd
-
drug development
-
analysis
-
synthesis
- 4.2.1.9
-
branched-chain
-
dhads
- fe-s
- acetolactate
-
isomeroreductase
- isobutanol
- 2-ketoisovalerate
- reductoisomerase
- 2,3-butanediol
-
bcaas
-
ketol-acid
-
ilvbncd
- drug development
- analysis
- synthesis
Reaction
Synonyms
2,3-dihydroxy acid hydrolyase, 2,3-dihydroxyisovalerate dehydratase, acetohydroxyacid dehydratase, AfIlv3A, AFUA_2G2410, alpha,beta-dihydroxy acid dehydratase, alpha,beta-dihydroxyisovalerate dehydratase, AT3G23940, DAD, dehydratase, dihydroxy acid, DHAD, dihydroxy acid dehydrase, dihydroxy acid dehydratase, dihydroxyacid dehydratase, H16_A2987, ilvC, IlvD, SsDHAD, VEG110, Vegetative protein 110
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Reference on EC 4.2.1.9 - dihydroxy-acid dehydratase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Myers, J.W.
Dihydroxy acid dehydrase: an enzyme involved in the biosynthesis of isoleucine and valine
J. Biol. Chem.
236
1414-1418
1961
Escherichia coli
Kanamori, M.; Wixom, R.L.
Studies in valine biosynthesis. V. Characteristics of the purified dihydroxyacid dehydratase from spinach leaves
J. Biol. Chem.
238
998-1005
1963
Spinacia oleracea
Altmiller, D.H.; Wagner, R.P.
Purification and properties of dihydroxy acid dehydratase from soluble and mitochondrial fractions of Neurospora crassa
Arch. Biochem. Biophys.
138
160-170
1970
Neurospora crassa
Wixom, R.L.; Garrett, J.L.; Fetzek, J.P.
A rapid determination of dihydroxyacid dehydratase activity in microbial cell suspensions
Anal. Biochem.
42
262-274
1971
Klebsiella aerogenes, Escherichia coli
Kiritani, K.; Wagner, R.P.
alpha,beta-Dihydroxy acid dehydratase (Neurospora crassa and spinach)
Methods Enzymol.
17A
755-764
1970
Neurospora crassa, Spinacia oleracea
-
Wixom, R.L.; Heinemann, M.A.; Semeraro, R.J.; Joseph, A.A.
Studies in valine biosynthesis. IX. The enzymes in photosynthetic and autotrophic bacteria
Biochim. Biophys. Acta
244
532-546
1971
Cupriavidus necator, Achromobacter ruhlandii, Chromatium sp., Acidovorax facilis, Paracoccus denitrificans, Cereibacter sphaeroides, Rhodospirillum rubrum, Chromatium sp. D
Cassady, W.E.; Leiter, E.H.; Bergquist, A.; Wagner, R.P.
Separation of mitochondrial membranes of Neurospora crassa. II. Submitochondrial localization of the isoleucine-valine biosynthetic pathway
J. Cell Biol.
53
66-72
1972
Neurospora crassa
Twarog, R.
Enzymes of the isoleucine-valine pathway in Acinetobacter
J. Bacteriol.
111
37-46
1972
Acinetobacter sp., Acinetobacter sp. 15150
Altmiller, D.H.
Neurospora mutants with mitochondria deficient in dihydroxy acid dehydratase. Properties of dihydroxy acid dehydratase from mutant strain 332
Biochem. Biophys. Res. Commun.
49
1000-1006
1972
Neurospora crassa
Armstrong, F.B.
Stereochemistry of the reductoisomerase and alpha,beta-dihydroxyacid dehydratase-catalysed steps in valine and isoleucine biosynthesis. Observation of a novel tertiary ketol rearrangement
J. Chem. Soc. Chem. Commun.
1974
351-352
1974
Salmonella enterica subsp. enterica serovar Typhimurium
-
Ryan, E.D.; Kohlhaw, G.B.
Subcellular localization of isoleucine-valine biosynthetic enzymes in yeast
J. Bacteriol.
120
631-637
1974
Saccharomyces cerevisiae
Armstrong, F.B.; Muller, U.S.; Reary, J.B.; Whitehouse, D.; Crout, D.H.G.
Stereoselectivity and stereospecificity of the alpha,beta-dihydroxyacid dehydratase from Salmonella typhimurium
Biochim. Biophys. Acta
498
282-293
1977
Salmonella enterica subsp. enterica serovar Typhimurium
Brown, O.R.; Yein, F.
Dihydroxyacid dehydratase: the site of hyperbaric oxygen poisoning in branch-chain amino acid biosynthesis
Biochem. Biophys. Res. Commun.
85
1219-1224
1978
Escherichia coli
Flint, D.H.; Emptage, M.H.
Dihydroxy acid dehydratase from spinach contains a [2Fe-2S] cluster
J. Biol. Chem.
263
3558-3564
1988
Spinacia oleracea
Kuo, C.F.; Mashino, T.; Fridovich, I.
An activity stain for dihydroxy-acid dehydratase
Anal. Biochem.
164
526-530
1987
Escherichia coli
Armstrong, F.B.; Lipscomb, E.L.; Crout, D.H.G.; Morgan, P.J.
Structure-activity studies with alpha,beta-dihydroxyacid dehydratase of Salmonella typhimurium
J. Chem. Soc. Perkin Trans.
1
691-696
1985
Salmonella enterica subsp. enterica serovar Typhimurium
-
Flint, D.H.; Tuminello, J.F.; Emptage, M.H.
The inactivation of Fe-S cluster containing hydro-lyases by superoxide
J. Biol. Chem.
268
22369-22376
1993
Escherichia coli
Flint, D.H.; Emptage, M.H.; Finnegan, M.G.; Fu, W.; Johnson, M.K.
The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase
J. Biol. Chem.
268
14732-14742
1993
Escherichia coli
Flint, D.H.; Smyk-Randall, E.; Tuminello, J.F.; Draczynska-Lusiak, B.; Brown, O.R.
The inactivation of dihydroxy-acid dehydratase in Escherichia coli treated with hyperbaric oxygen occurs because of the destruction of its Fe-S cluster, but the enzyme remains in the cell in a form that can be reactivated
J. Biol. Chem.
268
25547-25552
1993
Escherichia coli
Babu, B.N.; Brown, O.R.
Quantitative effects of redox-cycling chemicals on the oxidant-sensitive enzyme dihydroxy-acid dehydratase
Microbios
82
157-170
1995
Escherichia coli
Takabatake, T.; Hasegawa, M.; Nagano, T.; Hirobe, M.
Bacteriostatic effect of 4,7-dicyanobenzofurazan due to inactivation of 2,3-dihydroxyisovalerate dehydratase
Chem. Pharm. Bull.
40
1644-1646
1992
Escherichia coli
Matsumura, Y.; Imanaka, T.
Isolation and characterization of oxygen sensitive mutants of Escherichia coli
J. Ferment. Bioeng.
74
262-266
1992
Escherichia coli
-
Limberg, G.; Klaffke, W.; Thiem, J.
Conversion of aldonic acids to their corresponding 2-keto-3-deoxy-analogs by the non-carbohydrate enzyme dihydroxy acid dehydratase (DHAD)
Bioorg. Med. Chem.
3
487-494
1995
Spinacia oleracea
Flint, D.H.; Tuminello, J.F.; Miller, T.J.
Studies on the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase in escherichia coli crude extract. Isolation of O-acetylserine sulfhydrylases A and B and beta-cystathionase based on their ability to mobilize sulfur from cysteine and to participate in Fe-S cluster synthesis
J. Biol. Chem.
271
16053-16067
1996
Escherichia coli
Xing, R.; Whitman, W.B.
Characterization of enzymes of the branched-chain amino acid biosynthetic pathway in Methanococcus spp
J. Bacteriol.
173
2086-2092
1991
Methanococcus aeolicus, Methanococcus maripaludis, Methanococcus voltae
Smyk-Randall, E.M.; Brown, O.R.
A reverse-phase high-performance liquid chromatography assay for dihydroxy-acid dehydratase
Anal. Biochem.
164
434-438
1987
Escherichia coli
Pirrung, M.C.; Holmes, C.P.; Horowitz, D.M.; Nunn, D.S.
Mechanism and stereochemistry of alpha,beta-dihydroxyacid dehydratase
J. Am. Chem. Soc.
113
1020-1025
1991
Spinacia oleracea
-
Kim, S.; Lee, S.B.
Catalytic promiscuity in dihydroxy-acid dehydratase from the thermoacidophilic archaeon Sulfolobus solfataricus
J. Biochem.
139
591-596
2006
Saccharolobus solfataricus (Q97UB2), Saccharolobus solfataricus, Saccharolobus solfataricus P2 (Q97UB2)
Hyduke, D.R.; Jarboe, L.R.; Tran, L.M.; Chou, K.J.; Liao, J.C.
Integrated network analysis identifies nitric oxide response networks and dihydroxyacid dehydratase as a crucial target in Escherichia coli
Proc. Natl. Acad. Sci. USA
104
8484-8489
2007
Escherichia coli
Duan, X.; Yang, J.; Ren, B.; Tan, G.; Ding, H.
Reactivity of nitric oxide with the [4Fe-4S] cluster of dihydroxyacid dehydratase from Escherichia coli
Biochem. J.
417
783-789
2009
Escherichia coli
Holatko, J.; Elisakova, V.; Prouza, M.; Sobotka, M.; Nesvera, J.; Patek, M.
Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation
J. Biotechnol.
139
203-210
2009
Corynebacterium glutamicum
Ren, B.; Zhang, N.; Yang, J.; Ding, H.
Nitric oxide-induced bacteriostasis and modification of iron-sulphur proteins in Escherichia coli
Mol. Microbiol.
70
953-964
2008
Escherichia coli
Krause, F.S.; Blombach, B.; Eikmanns, B.J.
Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production
Appl. Environ. Microbiol.
76
8053-8061
2010
Corynebacterium glutamicum
Li, S.; Wen, J.; Jia, X.
Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression
Appl. Microbiol. Biotechnol.
91
577-589
2011
Corynebacterium glutamicum (Q8NQZ9), Corynebacterium glutamicum
Park, J.H.; Jang, Y.S.; Lee, J.W.; Lee, S.Y.
Escherichia coli W as a new platform strain for the enhanced production of L-valine by systems metabolic engineering
Biotechnol. Bioeng.
108
1140-1147
2011
Escherichia coli
Denina, I.; Paegle, L.; Prouza, M.; Holatko, J.; Patek, M.; Nesvera, J.; Ruklisha, M.
Factors enhancing L-valine production by the growth-limited L-isoleucine auxotrophic strain Corynebacterium glutamicum DeltailvA DeltapanB ilvNM13 (pECKAilvBNC)
J. Ind. Microbiol. Biotechnol.
37
689-699
2010
Corynebacterium glutamicum (Q8NQZ9), Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 (Q8NQZ9)
Singh, V.; Chandra, D.; Srivastava, B.S.; Srivastava, R.
Downregulation of Rv0189c, encoding a dihydroxyacid dehydratase, affects growth of Mycobacterium tuberculosis in vitro and in mice
Microbiology
157
38-46
2011
Escherichia coli, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Oliver, J.D.; Kaye, S.J.; Tuckwell, D.; Johns, A.E.; Macdonald, D.A.; Livermore, J.; Warn, P.A.; Birch, M.; Bromley, M.J.
The Aspergillus fumigatus dihydroxyacid dehydratase Ilv3A/IlvC is required for full virulence
PLoS ONE
7
e43559
2012
Aspergillus fumigatus
Carsten, J.M.; Philipp, A.; Sieber, V.
Characterization of recombinantly expressed dihydroxy-acid dehydratase from Sulfobus solfataricus - a key enzyme for the conversion of carbohydrates into chemicals
J. Biotechnol.
211
31-41
2015
Saccharolobus solfataricus (Q97UB2), Saccharolobus solfataricus, Saccharolobus solfataricus P2 (Q97UB2)
Lu, J.; Brigham, C.J.; Plassmeier, J.K.; Sinskey, A.J.
Characterization and modification of enzymes in the 2-ketoisovalerate biosynthesis pathway of Ralstonia eutropha H16
Appl. Microbiol. Biotechnol.
99
761-774
2015
Cupriavidus necator (Q0K7F8), Cupriavidus necator
Gao, H.; Azam, T.; Randeniya, S.; Couturier, J.; Rouhier, N.; Johnson, M.K.
Function and maturation of the Fe-S center in dihydroxyacid dehydratase from Arabidopsis
J. Biol. Chem.
293
4422-4433
2018
Arabidopsis thaliana (Q9LIR4)
Zhang, C.; Pang, Q.; Jiang, L.; Wang, S.; Yan, X.; Chen, S.; He, Y.
Dihydroxyacid dehydratase is important for gametophyte development and disruption causes increased susceptibility to salinity stress in Arabidopsis
J. Exp. Bot.
66
879-888
2015
Arabidopsis thaliana (Q9LIR4), Arabidopsis thaliana
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