4.1.3.1: isocitrate lyase
This is an abbreviated version!
For detailed information about isocitrate lyase, go to the full flat file.
Word Map on EC 4.1.3.1
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4.1.3.1
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interstrand
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malate
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lens
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eyes
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collamer
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postoperative
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intraocular
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crosslinks
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refract
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preoperative
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phakic
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acuity
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fanconi
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anemia
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posterior
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spherical
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uncorrected
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myopic
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vault
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fork
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diopter
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corneal
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astigmatism
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glyoxysomal
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fancd2
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pupil
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lymphocytopenia
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translesion
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logmar
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itaconate
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anaplerotic
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monoubiquitination
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psoralens
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monoadducts
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gaba-induced
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vision-threatening
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pupillary
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incisions
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hyperopic
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keratomileusis
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subcapsular
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lasik
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microbodies
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biomicroscopy
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drug development
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snellen
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medicine
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as-oct
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best-corrected
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sn-glycerol-3-phosphate
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spectacle
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molecular biology
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gelatinosa
- 4.1.3.1
-
interstrand
- malate
- lens
- eyes
-
collamer
-
postoperative
-
intraocular
-
crosslinks
-
refract
-
preoperative
-
phakic
-
acuity
-
fanconi
- anemia
-
posterior
-
spherical
-
uncorrected
-
myopic
-
vault
-
fork
-
diopter
- corneal
- astigmatism
- glyoxysomal
-
fancd2
- pupil
- lymphocytopenia
-
translesion
-
logmar
- itaconate
-
anaplerotic
-
monoubiquitination
- psoralens
-
monoadducts
-
gaba-induced
-
vision-threatening
-
pupillary
-
incisions
-
hyperopic
-
keratomileusis
-
subcapsular
-
lasik
- microbodies
-
biomicroscopy
- drug development
-
snellen
- medicine
-
as-oct
-
best-corrected
- sn-glycerol-3-phosphate
-
spectacle
- molecular biology
- gelatinosa
Reaction
Synonyms
AceA, acuD, citrate lyase, D-threo-isocitrate: glyoxylate lyase, EgGCE, FPICL1, ICL, ICL1, ICL2, isocitrase, isocitratase, isocitrate lyase, isocitrate lyase 1, isocitrate lyase 2, isocitric lyase, isocitritase, lyase, isocitrate, MtbIcl, PDP, petal death protein, SSO1333, threo-DS-Isocitrate glyoxylate-lyase
ECTree
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Purification
Purification on EC 4.1.3.1 - isocitrate lyase
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ammonium sulfate frationation, gel filtration, ion exchange chromatography (DEAE-cellulose)
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ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-25 gel filtration
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ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Resource Q column chromatography
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anion exchange chromatography (DEAE), immobilized metal ion affinity chromatography (Ni2+), gel filtration
ecombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
HiTrap Q FF ion exchange column chromatography, Superdex S-200 gel filtration, and HiTrap Q HP ion exchange column chromatography
immobilized metal ion affinity chromatography (Ni2+), tags removed
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nicke affinity chromatography, cleavage of the His-tag by thrombin, and dialysis
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recombinant protein from cell lysate by Ni-NTA metal-affinity chromatography (elution: 400 mM imidazole) and gel filtration on Superdex 200HR 10/300 column
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to homogeneity, purification of ICL1 is 123fold with a yield of 5.7%, purification degree and yield of ICL2 are 110 and 5.7%, respectively
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