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Literature summary for 4.1.3.1 extracted from

  • Mackintosh, C.; Nimmo, H.G.
    Purification and regulatory properties of isocitrate lyase from Escherichia coli ML308 (1988), Biochem. J., 250, 25-31.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.1

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate
-
 Escherichia coli
3-phosphoglycerate
-
 Escherichia coli
Cl-
-
 Escherichia coli
glycolate
-
 Escherichia coli
phosphate
-
 Escherichia coli
phosphoenolpyruvate
-
 Escherichia coli
SO42-
-
 Escherichia coli
succinate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.062
-
 Ds-isocitrate pH 7.3 Escherichia coli
0.13
-
 glyoxylate
-
 Escherichia coli
0.59
-
 succinate
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44700
-
 4 * 44700, SDS-PAGE Escherichia coli
177000
-
 gel filtration Escherichia coli
180000
-
 sedimentation equilibrium centrifugation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37.5
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, at least 6 months, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate
-
 Escherichia coli succinate + glyoxylate
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 44700, SDS-PAGE Escherichia coli