Information on EC 4.1.3.1 - isocitrate lyase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
4.1.3.1
-
RECOMMENDED NAME
GeneOntology No.
isocitrate lyase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
isocitrate = succinate + glyoxylate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
-
-
-
-
elimination
-
-
of an oxo-acid, C-C bond cleavage
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
citric acid cycle
-
-
Glyoxylate and dicarboxylate metabolism
-
-
glyoxylate cycle
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
TCA cycle IV (2-oxoglutarate decarboxylase)
-
-
TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase)
-
-
SYSTEMATIC NAME
IUBMB Comments
isocitrate glyoxylate-lyase (succinate-forming)
The isomer of isocitrate involved is (1R,2S)-1-hydroxypropane-1,2,3-tricarboxylate [3].
CAS REGISTRY NUMBER
COMMENTARY hide
9045-78-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC46645
-
-
Manually annotated by BRENDA team
strain N402
-
-
Manually annotated by BRENDA team
strain N402
-
-
Manually annotated by BRENDA team
gene icl
UniProt
Manually annotated by BRENDA team
gene icl
UniProt
Manually annotated by BRENDA team
thermophilic
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strains 1026b and DD503
-
-
Manually annotated by BRENDA team
Candida albicans ATCC 10231
-
-
-
Manually annotated by BRENDA team
Candida brassicae
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain NRC 1004
UniProt
Manually annotated by BRENDA team
strain NRC 1004
UniProt
Manually annotated by BRENDA team
gene aceA
-
-
Manually annotated by BRENDA team
gene aceA
-
-
Manually annotated by BRENDA team
cucumber
-
-
Manually annotated by BRENDA team
Y7426
-
-
Manually annotated by BRENDA team
Y7426
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Escherichia coli K-12 W3110
strain K-12 W3110
-
-
Manually annotated by BRENDA team
SM-ZK
Swissprot
Manually annotated by BRENDA team
strain TYP-6137, formerly named Tyromyces palustris
EMBL
Manually annotated by BRENDA team
cotton
Uniprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
sunflower
-
-
Manually annotated by BRENDA team
Klicl1 deletion mutant
-
-
Manually annotated by BRENDA team
Lupinus sp.
-
-
-
Manually annotated by BRENDA team
Magnaporthe grisea Guy 11
strain Guy 11
-
-
Manually annotated by BRENDA team
Mycobacterium avium My 330/88
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Mycobacterium kansasii My 235/80
-
-
-
Manually annotated by BRENDA team
1-2c
-
-
Manually annotated by BRENDA team
Mycobacterium tuberculosis My 331/88
-
-
-
Manually annotated by BRENDA team
ssp. indica cv Guangluai
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 13525
-
-
Manually annotated by BRENDA team
wild-type: KT2442, mutant: KT217, poly(3-hydroxyalkanoate)-overproducing mutant in which aceA gene is knocked out
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
serovar typhimurium
-
-
Manually annotated by BRENDA team
strain Rm5000
-
-
Manually annotated by BRENDA team
NRRL 3585
SwissProt
Manually annotated by BRENDA team
NRRL 3585
SwissProt
Manually annotated by BRENDA team
strain DSM 639
-
-
Manually annotated by BRENDA team
CBS 119456
-
-
Manually annotated by BRENDA team
isolate ATCC 95640
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
conserved residues in the subfamily 3 signature of ICL-Pa play important roles in catalysis and thermostability and are likely associated with the catalytic loop structural conformation. Three-dimensional structural homology modeling of ICL-Pa wild-type and mutants, structure comparisons, active site modeling, overview. Residue N214 plays an essential role in ICL-Pa catalytic activity
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1S,2S)-1-hydroxypropane-1,2,3-tricarboxylate
succinate + glyoxylate
show the reaction diagram
-
reversible aldol cleavage
glyoxylate shunt
-
r
(2R)-2-ethyl malate
acetate + 2-oxobutanoate
show the reaction diagram
-
-
-
?
(2R)-2-methyl malate
pyruvate + acetate
show the reaction diagram
-
-
-
?
(2R)-ethyl-(3S)-methyl malate
2-oxobutanoate + propanoate
show the reaction diagram
-
-
-
?
(2R)-isobutyl-(3S)-methyl malate
4-methyl-2-oxopentanoate + propanoate
show the reaction diagram
-
-
-
?
(2R)-propyl-(3S)-methyl malate
4-methyl-2-oxopentanoate + propanoate
show the reaction diagram
-
-
-
?
(2R,3R:2S,3S)-2-methyl-isocitrate
succinate + pyruvate
show the reaction diagram
-
-
-
?
(2R,3S)-2,3-dimethyl malate
pyruvate + propanoate
show the reaction diagram
-
-
-
?
(2R,3S)-isocitrate
succinate + glyoxylate
show the reaction diagram
(2R,3S:2S,3R)-2-methyl-isocitrate
succinate + pyruvate
show the reaction diagram
(2S)-2-methyl malate
pyruvate + acetate
show the reaction diagram
kcat below 1x10-5
-
-
?
(R)-malate
glyoxylate + acetate
show the reaction diagram
kcat below 1x10-5
-
-
?
(S)-malate
glyoxylate + acetate
show the reaction diagram
kcat below 1x10-5
-
-
?
acetyl-CoA + glyoxylate + H2O
malate + CoA
show the reaction diagram
D,L-isocitrate
succinate + glyoxylate
show the reaction diagram
-
-
-
-
?
D-isocitrate
succinate + glyoxylate
show the reaction diagram
DL-isocitrate
succinate + glyoxylate
show the reaction diagram
-
-
-
-
?
isocitrate
?
show the reaction diagram
isocitrate
glyoxylate + succinate
show the reaction diagram
isocitrate
succinate + glyoxylate
show the reaction diagram
isocitrate + phenylhydrazine
? + glyoxylate phenylhydrazone
show the reaction diagram
-
-
-
?
oxalacetate
oxalate + acetate
show the reaction diagram
-
-
-
?
succinate + glyoxylate
isocitrate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(1S,2S)-1-hydroxypropane-1,2,3-tricarboxylate
succinate + glyoxylate
show the reaction diagram
-
reversible aldol cleavage
glyoxylate shunt
-
r
isocitrate
?
show the reaction diagram
isocitrate
succinate + glyoxylate
show the reaction diagram
additional information
?
-
-
Mycobacterium tuberculosis isocitrate lyases are catalytically bifunctional isocitrate and methylisocitrate lyases, EC 4.1.3.1 and EC 4.1.3.30, required for growth on even and odd chain fatty acids
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
5 mM, 90% inhibition of Mg2+-activated enzyme, interaction with catalytic domain induces partial unfolding (revealed by thermal denaturation, far-UV circular dichroism, fluorescence spectra, and glutaraldehyde crosslinking)
Zn2+
-
5 mM, 90% inhibition of Mg2+-activated enzyme, interaction with catalytic domain induces partial unfolding (revealed by thermal denaturation, far-UV circular dichroism, ANS fluorescence spectra, and glutaraldehyde crosslinking)
additional information
-
no significant change in enzyme activity after incubation with Co2+, Pb2+, Fe2+, Cu2+, Cd2+, Na+, K+, Cl-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2R,6E)-8-(2,5-dihydroxyphenyl)-2-hydroxy-6-methyl-1-[[(1S,4aS,8aS)-2,5,5,8a-tetramethyl-1,4,4a,5,6,7,8,8a-octahydronaphthalen-1-yl]methyl]oct-6-en-1-yl hydrogen sulfate
-
-
(1S,4bS,10aR,12aS)-10a-[(acetyloxy)methyl]-1-formyl-4b,7,7,12a-tetramethyl-1,4,4a,4b,5,6,6a,7,8,9,10,10a,10b,11,12,12a-hexadecahydrochrysene-2-carboxylic acid
-
scarlarane sesterterpene, isolated from Smenospongia sp.
(2-bromo-4,5-dihydroxybenzyl)(2,3-dibromo-4,5-dihydroxybenzyl) ether
-
-
(2-bromo-4,5-dihydroxyphenyl)(3,4-dihydroxyphenyl) ether
-
-
(2E,6E)-9-[(2R)-6-hydroxy-2,8-dimethyl-3,4-dihydro-2H-chromen-2-yl]-2,6-dimethylnona-2,6-dienal
-
-
(2E,7E)-10-[(2R)-6-hydroxy-2,8-dimethyl-3,4-dihydro-2H-chromen-2-yl]-3,7-dimethyl-2-(2-methylprop-1-en-1-yl)deca-2,7-dienal
-
-
(2R)-2-[(3E,7E)-9-(hydroxymethyl)-4,8,11-trimethyldodeca-3,7,10-trien-1-yl]-2,8-dimethyl-3,4-dihydro-2H-chromen-6-ol
-
-
(3-bromo-4,5-dihydroxyphenyl)(2,3-dibromo-4,5-dihydroxyphenyl)methanone
-
-
(4R,5R)-4-[(2E)-5-[(2R)-6-hydroxy-2,8-dimethyl-3,4-dihydro-2H-chromen-2-yl]-2-methylpent-2-en-1-yl]-2-methyl-5-(2-methylprop-1-en-1-yl)cyclopent-2-en-1-one
-
-
(4S,5bR,11aS,13aS,13bR)-4-hydroxy-5b,8,8,11a,13a-pentamethyl-1,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a,13b-octadecahydrochryseno[1,2-c]furan-1-yl acetate
-
scarlarane sesterterpene, isolated from Smenospongia sp.
(4S,5bS,11aR,13aS,13bR)-11a-formyl-4-hydroxy-5b,8,8,13a-tetramethyl-1,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a,13b-octadecahydrochryseno[1,2-c]furan-1-yl acetate
-
scarlarane sesterterpene, isolated from Smenospongia sp.
(4S,5bS,11aR,13aS,13bR)-11a-[(acetyloxy)methyl]-5b,8,8,13a-tetramethyl-1,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a,13b-octadecahydrochryseno[1,2-c]furan-1,4-diyl diacetate
-
scarlarane sesterterpene, isolated from Smenospongia sp.
(5bS,11aR,13aS,13bR)-11a-(hydroxymethyl)-5b,8,8,13a-tetramethyl-3-oxo-1,3,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a,13b-octadecahydrochryseno[1,2-c]furan-1-yl acetate
-
scarlarane sesterterpene, isolated from Smenospongia sp.
(5E)-5-[(2R,6E,10E)-13-furan-3-yl-2,6,10-trimethyltrideca-6,10-dien-1-ylidene]-4-hydroxy-3-methylfuran-2(5H)-one
-
linear furanosesterterpene, isolated from Smenospongia sp.
(5Z)-4-(3,5-dibromo-4-hydroxyphenyl)-3-[(S)-(3,5-dibromo-4-methoxyphenyl)(hydroxy)methyl]-5-(4-methoxybenzylidene)furan-2(5H)-one
i.e. cadiolide H, purified from the ascidian Synoicum sp.
(5Z)-4-(3-bromo-4-hydroxyphenyl)-3-(3,5-dibromo-4-hydroxybenzoyl)-5-(3,5-dibromo-4-hydroxybenzylidene)furan-2(5H)-one
i.e. cadiolide E, purified from the ascidian Synoicum sp.
(5Z)-5-(3-bromo-4-hydroxybenzylidene)-4-(3-bromo-4-hydroxyphenyl)-3-[(S)-(3,5-dibromo-4-methoxyphenyl)(hydroxy)methyl]furan-2(5H)-one
i.e. cadiolide G, purified from the ascidian Synoicum sp.
(5Z)-5-[(2R,5Z,9Z)-13-furan-3-yl-2,6,10-trimethyltrideca-5,9-dien-1-ylidene]-4-hydroxy-3-methylfuran-2(5H)-one
-
linear furanosesterterpene, isolated from Smenospongia sp.
(E)-2-(3-methylnon-2-enyl)benzene-1,4-diol
-
-
(E)-2-(4-hydroxy-3-methylbut-2-enyl)benzene-1,4-diol
-
-
(E)-2-(9-hydroxy-3-methylnon-2-enyl)benzene-1,4-diol
-
-
1,2-bis(5-hydroxy-1H-indol-3-yl)ethane-1,2-dione
-
-
1-(2-aminoethyl)-1H-indol-6-ol
-
-
1-(3-methoxyphenyl)-2,3,4,9-tetrahydro-1H-b-carboline
-
-
1-(4-ethylpiperazin-1-yl)-3-nitropropan-1-one
-
-
1-(4-methoxyphenyl)-2,3,4,9-tetrahydro-1H-b-carboline
-
-
1-(naphthalen-2-yl)-2,3,4,9-tetrahydro-1H-b-carboline
-
-
1-carboxy-6-hydroxy-3,4-dihydro-beta-carboline
-
weak inhibition, isolated from marine sponge Hyrtios sp. (Thorectidae family)
1-cyclopropyl-6-fluoro-7-[4-(3-nitropropanoyl)piperazin-1-yl]-4-oxo-1,4-dihydroquinoline-3-carboxylic acid
-
-
1-cyclopropyl-7-(3,5-dimethyl-4-(3-nitropropanoyl)piperazin-1-yl)-6-fluoro-8-methoxy-4-oxo-1,4-dihydroquinoline-3-carboxylic acid
-
-
1-cyclopropyl-7-[3,5-dimethyl-4-(3-nitropropanoyl)piperazin-1-yl]-6-fluoro-8-methoxy-4-oxo-1,4-dihydroquinoline-3-carboxylic acid
-
a fluoroquinolone derivative and structural analogue of succinate
-
1-ethyl-6,8-difluoro-7-[3-methyl-4-(3-nitropropanoyl)piperazin-1-yl]-4-oxo-1,4-dihydroquinoline-3-carboxylic acid
-
-
1-ethyl-6-fluoro-7-[4-(3-nitropropanoyl)piperazin-1-yl]-4-oxo-1,4-dihydroquinoline-3-carboxylic acid
-
-
12-deacetoxy-23-acetoxy-19-O-acetylscalarin
-
cytotoxic, isolated from Smenospongia sp.
12-deacetoxy-23-acetoxyscalarin
-
cytotoxic, isolated from Smenospongia sp.
12-deacetoxy-23-hydroxyheteronemin
-
cytotoxic, isolated from Smenospongia sp.
2,2',3,3'-tetrabromo-4,4',5,5'-tetrahydroxydiphenylmethane
-
-
2,2',3-tribromo-3',4,4',5-tetrahydroxy-6'-hydroxymethyldiphenylmethane
-
-
2,2',3-tribromo-4,4',5,5'-tetrahydroxybibenzyl
-
-
2,2'-dibromo-4,4',5,5'-tetrahydroxybibenzyl
-
-
2,3-dibromo-4,5-dihydroxybenzyl alcohol
-
-
2,3-dibromo-4,5-dihydroxybenzyl methyl ether
-
-
2-(3-methylbut-2-enyl)benzene-1,4-diol
-
-
2-(4-chloro-2-methoxybenzamido)-5-(trifluoromethyl)phenyl 4-chloro-2-methoxybenzoate
2-(4-chloro-2-methoxyphenyl)-6-(trifluoromethyl)benzo[d]oxazole
2-(5-chloro-2-methoxybenzamido)-5-(trifluoromethyl)phenyl 5-chloro-2-methoxybenzoate
2-(5-chloro-2-methoxyphenyl)-6-(trifluoromethyl)benzo[d]oxazole
2-allylbenzene-1,4-diol
-
-
2-hydroxy-1-(5-hydroxy-1H-indol-3-yl)ethanone
-
-
2-mercaptoethanol
2-oxoglutarate
2-[(2E,7R,8R)-7,8-dihydroxy-3-methyl-9-[(1S,4aS,8aS)-2,5,5,8a-tetramethyl-1,4,4a,5,6,7,8,8a-octahydronaphthalen-1-yl]non-2-en-1-yl]benzene-1,4-diol
-
-
3,5-dibromo-4-hydroxyphenylethylamine
-
-
3-bromo-4-(2,3-dibromo-4,5-dihydroxybenzyl)-5-methoxymethylpyrocatechol
-
-
3-nitro-1-(4-phenylpiperazin-1-yl)propan-1-one
-
-
3-nitro-1-[4-[3-(trifluoromethyl)phenyl]piperazin-1-yl]propan-1-one
-
-
3-nitropropionate
3-nitropropionic acid
3-phosphoglycerate
4,4'-(oxydimethanediyl)bis(5,6-dibromobenzene-1,2-diol)
-
-
4,4'-ethane-1,2-diyldibenzene-1,2-diol
-
-
4,4'-methanediylbis(5,6-dibromobenzene-1,2-diol)
-
-
4,4'-methanediylbis(5-bromobenzene-1,2-diol)
-
-
4,5-dichloro-2-(5-chloro-2-methoxybenzamido)phenyl 5-chloro-2-methoxybenzoate
4-chloro-N-(2-hydroxy-4-(trifluoromethyl)phenyl)-2-methoxybenzamide
4-chloro-N-(2-hydroxy-4-(trifluoromethyl)phenyl)-2-methoxybenzothioamide
4-chloro-N-(2-hydroxy-5-(trifluoromethyl)phenyl)-2-methoxybenzamide
4-chloro-N-(2-hydroxy-5-(trifluoromethyl)phenyl)-2-methoxybenzothioamide
4-chloro-N-(4,5-dichloro-2-hydroxyphenyl)-2-methoxybenzamide
4-chloro-N-(4,5-dichloro-2-hydroxyphenyl)-2-methoxybenzothioamide
4-chloro-N-(4-chloro-2-hydroxyphenyl)-2-methoxybenzamide
4-chloro-N-(4-chloro-2-hydroxyphenyl)-2-methoxybenzothioamide
4-chloro-N-(5-chloro-2-hydroxyphenyl)-2-methoxybenzamide
4-chloro-N-(5-chloro-2-hydroxyphenyl)-2-methoxybenzothioamide
4-chloromercuribenzoate
-
complete inactivation at 0.001 mM
4-hydroxy-9-deoxoidiadione
-
cytotoxic, linear furanosesterterpene, isolated from Smenospongia sp.
5,6-dichloro-2-(4-chloro-2-methoxyphenyl)benzo[d]oxazole
5,6-dichloro-2-(5-chloro-2-methoxyphenyl)benzo[d]oxazole
5-bromo-2-(4-chloro-2-methoxyphenyl)benzo[d]oxazole
5-bromo-2-(5-chloro-2-methoxyphenyl)benzo[d]oxazole
5-chloro-2-(4-chloro-2-methoxybenzamido)phenyl 4-chloro-2-methoxybenzoate
5-chloro-2-(5-chloro-2-methoxyphenyl)benzo[d]oxazole
5-chloro-N-(2-hydroxy-4-(trifluoromethyl)phenyl)-2-methoxybenzamide
5-chloro-N-(2-hydroxy-4-(trifluoromethyl)phenyl)-2-methoxybenzothioamide
5-chloro-N-(2-hydroxy-5-(trifluoromethyl)phenyl)-2-methoxybenzamide
5-chloro-N-(2-hydroxy-5-(trifluoromethyl)phenyl)-2-methoxybenzothioamide
5-chloro-N-(4,5-dichloro-2-hydroxyphenyl)-2-methoxybenzamide
5-chloro-N-(4,5-dichloro-2-hydroxyphenyl)-2-methoxybenzothioamide
5-chloro-N-(4-chloro-2-hydroxyphenyl)-2-methoxybenzamide
5-chloro-N-(4-chloro-2-hydroxyphenyl)-2-methoxybenzothioamide
5-chloro-N-(5-chloro-2-hydroxyphenyl)-2-methoxybenzamide
5-chloro-N-(5-chloro-2-hydroxyphenyl)-2-methoxybenzothioamide
5-hydroxy-1H-indole-3-carbaldehyde
-
-
5-hydroxy-1H-indole-3-carboxylic acid methyl ester
-
weak inhibition, isolated from marine sponge Hyrtios sp. (Thorectidae family)
5-hydroxyindole-3-carbaldehyde
-
moderate inhibition, isolated from marine sponge Hyrtios sp. (Thorectidae family)
6-bromo-2-(4-chloro-2-methoxyphenyl)benzo[d]oxazole
6-bromo-2-(5-chloro-2-methoxyphenyl)benzo[d]oxazole
6-chloro-2-(4-chloro-2-methoxyphenyl)benzo[d]oxazole
6-hydroxy-4,9-dihydro-3H-beta-carboline-1-carboxylic acid
-
-
ascorbate
-
enzyme is inhibited by an ascorbate plus Fe2+ system under aerobic conditions,16% residual activity at 2 mM ascorbate/0.02 mM Fe2+. Inactivation requires hydrogen peroxide, and can be prevented by catalase, EDTA, Mg2+, isocitrate, 20 mM glutathione, 20 mM dithiothreitol or 40 mM L-cysteine
ATP
-
35% inhibition at 2 mM
bis(2,3-dibromo-4,5-dihydroxyphenyl) ether
-
-
bis(2-bromo-4,5-dihydroxyphenyl) ether
-
-
bis(3,4-dihydroxyphenyl)methanone
-
-
bis(3-bromo-4,5-dihydroxyphenyl)methanone
-
-
citrate
cysteine
-
-
D-fructose-6-phosphate
-
-
D-Glucose-6-phosphate
-
-
dimethyl (2Z)-2-(3,5-dibromo-4-hydroxybenzoyl)-3-(3,5-dibromo-4-hydroxyphenyl)but-2-enedioate
i.e. synoilide A, purified from the ascidian Synoicum sp.
dimethyl (2Z)-2-(3-bromo-4-hydroxyphenyl)-3-(3,5-dibromo-4-hydroxybenzoyl)but-2-enedioate
i.e. synoilide B, purified from the ascidian Synoicum sp.
Dithionitrobenzoate
-
-
dithiothreitol
-
-
DL-penicillamin
-
-
Fe2+
-
enzyme is inhibited by an ascorbate plus Fe2+ system under aerobic conditions,16% residual activity at 2 mM ascorbate/0.02 mM Fe2+. Inactivation requires hydrogen peroxide, and can be prevented by catalase, EDTA, Mg2+, isocitrate, 20 mM glutathione, 20 mM dithiothreitol or 40 mM L-cysteine
fructose-1,6-bisphosphate
fumarate
Glucose-1-phosphate
-
-
glucose-6-phosphate
glutathione
glycolate
glyoxylate
GSSG
-
ICL can be inactivated by glutathionylation and reactivated by glutaredoxin after reduced dithiothreitol treatment, whereas thioredoxin does not appear to regulate ICL activity
halisulfate 1
-
-
halisulfate 5
-
-
HPO42-
hydrohalisulfate 1
-
-
Hydroxymalonate
hyrtiosin A
-
moderate inhibition, isolated from marine sponge Hyrtios sp. (Thorectidae family)
hyrtiosin B
-
strong inhibition, isolated from marine sponge Hyrtios sp. (Thorectidae family)
iodoacetate
Itaconate
Itaconic acid
itaconic anhydride
-
-
malate
Maleate
malonate
methyl (1R,4bS,10aR,12aS)-10a-[(acetyloxy)methyl]-1-formyl-4b,7,7,12a-tetramethyl-1,4,4a,4b,5,6,6a,7,8,9,10,10a,10b,11,12,12a-hexadecahydrochrysene-2-carboxylate
-
scarlarane sesterterpene, isolated from Smenospongia sp.
methyl (1S,4bS,10aR,12aS)-10a-[(acetyloxy)methyl]-1-formyl-4b,7,7,12a-tetramethyl-1,4,4a,4b,5,6,6a,7,8,9,10,10a,10b,11,12,12a-hexadecahydrochrysene-2-carboxylate
-
scarlarane sesterterpene, isolated from Smenospongia sp.
methyl 2-[bis(3,5-dibromo-4-hydroxyphenyl)methylidene]-4-(3,5-dibromo-4-hydroxyphenyl)-5-oxo-2,5-dihydrofuran-3-carboxylate
i.e. cadiolide I, purified from the ascidian Synoicum sp.
methylenesuccinate
methylmalonate
-
50% inhibition at 15 mM
N'1-[(4-nitrophenyl)methylene]-2-[3-(4-bromo-2-fluorobenzyl)-4-oxo-1,2,3,4-tetrahydro-1-phthalazinyl]ethanohydrazide
-
is the most active compound
N-(4-bromo-2-hydroxyphenyl)-4-chloro-2-methoxybenzamide
N-(4-bromo-2-hydroxyphenyl)-4-chloro-2-methoxybenzothioamide
N-(4-bromo-2-hydroxyphenyl)-5-chloro-2-methoxybenzamide
N-(4-bromo-2-hydroxyphenyl)-5-chloro-2-methoxybenzothioamide
N-(4-bromo-3-methylphenyl)-3-nitropropanamide
-
-
N-(4-bromophenyl)-3-nitropropanamide
-
-
N-(4-chlorophenyl)-3-nitropropanamide
-
-
N-(5-bromo-2-hydroxyphenyl)-4-chloro-2-methoxybenzamide
N-(5-bromo-2-hydroxyphenyl)-4-chloro-2-methoxybenzothioamide
N-(5-bromo-2-hydroxyphenyl)-5-chloro-2-methoxybenzamide
N-(5-bromo-2-hydroxyphenyl)-5-chloro-2-methoxybenzothioamide
N-ethylmaleimide
oxalacetate
oxalate
oxaloacetate
-
89% inhibition at 10 mM
p-chloromercuribenzoate
p-hydroxymercuribenzoate
-
complete inhibition at 0.016 mM
phosphate
phosphoenolpyruvate
pyruvate
serotonin
-
moderate inhibition, isolated from marine sponge Hyrtios sp. (Thorectidae family)
sodium (2S)-5-furan-3-yl-2-[(1R)-1-hydroxy-2-[(1S,2R,4aR,8aR)-2-methyl-5-methylidenedecahydronaphthalen-1-yl]ethyl]pentyl sulfate
-
-
succinate
sulfhydryl compounds
Tartrate
threo-DL-homoisocitrate
-
-
-
Urea
-
partial inactivation
XHD-1
-
extract of Illicium verum
-
XHD-2
-
extract of Zingiber officinale
-
Zn2+
Candida brassicae
-
-
[(5bS,11aR,13aS)-5b,8,8,13a-tetramethyl-5,5a,5b,6,7,7a,8,9,10,11,11b,12,13,13a-tetradecahydrochryseno[1,2-c]furan-11a(4H)-yl]methanol
-
scarlarane sesterterpene, isolated from Smenospongia sp.
[(5bS,11aR,13aS)-5b,8,8,13a-tetramethyl-5,5a,5b,6,7,7a,8,9,10,11,11b,12,13,13a-tetradecahydrochryseno[1,2-c]furan-11a(4H)-yl]methyl acetate
-
scarlarane sesterterpene, isolated from Smenospongia sp.
[(5bS,11aR,13aS,13bR)-1-methoxy-5b,8,8,13a-tetramethyl-1,5,5a,5b,6,7,7a,8,9,10,11,11b,12,13,13a,13b-hexadecahydrochryseno[1,2-c]furan-11a(3H)-yl]methanol
-
scarlarane sesterterpene, isolated from Smenospongia sp.
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
acetate
-
activity of the ICL promoter is significantly upregulated during growth on acetate
acetyl-CoA
cysteine
dithioerythritol
Candida brassicae
-
increase of activity
dithiothreitol
glutaredoxin 1
-
reactivation of GSSG-treated ICL in the presence of glutaredoxin 1 within 20 min
-
glutathione
L-cysteine
-
45% increase of activity at 1 mM
Na2S2O4
Lupinus sp.
-
increase of activity
Reducing agents
Lupinus sp.
-
increase of activity
-
sulfhydryl compounds
thioglycolate
Lupinus sp.
-
increase of activity
thiol compounds
additional information