Cloned (Comment) | Organism |
---|---|
expresssion in Escherichia coli | Dianthus caryophyllus |
Protein Variants | Comment | Organism |
---|---|---|
C144A | mutant with no enzyme activity | Dianthus caryophyllus |
D79A | mutant with 1000fold reduced turnover rate | Dianthus caryophyllus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
(2R,3S)-2,3-dimethyl malate | pH 7.5, 25°C | Dianthus caryophyllus | |
0.029 | - |
(2R,3S)-2,3-dimethyl malate | pH 7.5, 25°C | Dianthus caryophyllus | |
0.042 | - |
(2R,3S)-isocitrate | pH 7.5, 25°C | Dianthus caryophyllus | |
0.069 | - |
(2R,3S:2S,3R)-2-methyl-isocitrate | pH 7.5, 25°C | Dianthus caryophyllus | |
0.098 | - |
(2R)-propyl-(3S)-methyl malate | pH 7.5, 25°C | Dianthus caryophyllus | |
0.104 | - |
(2R,3R:2S,3S)-2-methyl-isocitrate | pH 7.5, 25°C | Dianthus caryophyllus | |
0.13 | - |
oxalacetate | pH 7.5, 25°C | Dianthus caryophyllus | |
0.29 | - |
(2R)-2-methyl malate | pH 7.5, 25°C | Dianthus caryophyllus | |
0.45 | - |
(2R)-ethyl-(3S)-methyl malate | pH 7.5, 25°C | Dianthus caryophyllus | |
0.53 | - |
(2R)-ethyl-(3S)-methyl malate | pH 7.5, 25°C | Dianthus caryophyllus | |
1.1 | - |
(2R)-2-ethyl malate | pH 7.5, 25°C | Dianthus caryophyllus | |
8 | - |
(2R)-isobutyl-(3S)-methyl malate | pH 7.5, 25°C | Dianthus caryophyllus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activating | Dianthus caryophyllus | |
Fe2+ | activating | Dianthus caryophyllus | |
Mg2+ | activating oxalacetate hydrolase activity | Dianthus caryophyllus | |
Mn2+ | activating oxalacetate hydrolase activity | Dianthus caryophyllus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE | Dianthus caryophyllus |
133000 | - |
gel filtration | Dianthus caryophyllus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dianthus caryophyllus | Q05957 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Dianthus caryophyllus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R)-2-ethyl malate | - |
Dianthus caryophyllus | acetate + 2-oxobutanoate | - |
? | |
(2R)-2-methyl malate | - |
Dianthus caryophyllus | pyruvate + acetate | - |
? | |
(2R)-ethyl-(3S)-methyl malate | - |
Dianthus caryophyllus | 2-oxobutanoate + propanoate | - |
? | |
(2R)-isobutyl-(3S)-methyl malate | - |
Dianthus caryophyllus | 4-methyl-2-oxopentanoate + propanoate | - |
? | |
(2R)-propyl-(3S)-methyl malate | - |
Dianthus caryophyllus | 4-methyl-2-oxopentanoate + propanoate | - |
? | |
(2R,3R:2S,3S)-2-methyl-isocitrate | - |
Dianthus caryophyllus | succinate + pyruvate | - |
? | |
(2R,3S)-2,3-dimethyl malate | - |
Dianthus caryophyllus | pyruvate + propanoate | - |
? | |
(2R,3S)-isocitrate | - |
Dianthus caryophyllus | succinate + glyoxylate | - |
? | |
(2R,3S:2S,3R)-2-methyl-isocitrate | - |
Dianthus caryophyllus | succinate + pyruvate | - |
? | |
(2S)-2-methyl malate | kcat below 1x10-5 | Dianthus caryophyllus | pyruvate + acetate | - |
? | |
(R)-malate | kcat below 1x10-5 | Dianthus caryophyllus | glyoxylate + acetate | - |
? | |
(S)-malate | kcat below 1x10-5 | Dianthus caryophyllus | glyoxylate + acetate | - |
? | |
oxalacetate | - |
Dianthus caryophyllus | oxalate + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 35000, SDS-PAGE | Dianthus caryophyllus |
Synonyms | Comment | Organism |
---|---|---|
PDP | - |
Dianthus caryophyllus |
petal death protein | - |
Dianthus caryophyllus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
(2R,3S)-2-ethyl-3-methyl malate | Dianthus caryophyllus |