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Literature summary for 4.1.3.1 extracted from
- Gene cloning of cold-adapted isocitrate lyase from a psychrophilic bacterium, Colwellia psychrerythraea, and analysis of amino acid residues involved in cold adaptation of this enzyme (2008), Extremophiles, 12, 107-117.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Top10 cells | Colwellia psychrerythraea |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A214S | increased thermostability and low specific activity at low temperature | Colwellia psychrerythraea |
| A231E | more thermolabile than the wild type enzyme | Colwellia psychrerythraea |
| A341N | decreased activity compared to the wild type enzyme | Colwellia psychrerythraea |
| F333L | very low activity | Colwellia psychrerythraea |
| Q47K | activity similar to the wild type enzyme | Colwellia psychrerythraea |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58131 | - |
4 * 58131, calculated from amino acid sequence | Colwellia psychrerythraea |
| 60000 | - |
4 * 60000, gel filtration | Colwellia psychrerythraea |
| 240000 | - |
gel filtration | Colwellia psychrerythraea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colwellia psychrerythraea | A8C5K8 | - |
- |
| Colwellia psychrerythraea NRC 1004 | A8C5K8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | - |
Colwellia psychrerythraea | succinate + glyoxylate | - |
? |  |
| isocitrate | - |
Colwellia psychrerythraea NRC 1004 | succinate + glyoxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 58131, calculated from amino acid sequence | Colwellia psychrerythraea |
| homotetramer | 4 * 60000, gel filtration | Colwellia psychrerythraea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ICL | - |
Colwellia psychrerythraea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Colwellia psychrerythraea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
the enzyme is rapidly inactivated at 30°C | Colwellia psychrerythraea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13.3 | - |
isocitrate | mutant enzyme A341N, at 25°C | Colwellia psychrerythraea | |
| 26.6 | - |
isocitrate | mutant enzyme Q47K, at 25°C | Colwellia psychrerythraea | |
| 28.5 | - |
isocitrate | wild type enzyme, at 25°C | Colwellia psychrerythraea | |
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