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4.1.3.1: isocitrate lyase

This is an abbreviated version!
For detailed information about isocitrate lyase, go to the full flat file.

Word Map on EC 4.1.3.1

Reaction

isocitrate
=
succinate
 +
glyoxylate

Synonyms

AceA, acuD, citrate lyase, D-threo-isocitrate: glyoxylate lyase, EgGCE, FPICL1, ICL, ICL1, ICL2, isocitrase, isocitratase, isocitrate lyase, isocitrate lyase 1, isocitrate lyase 2, isocitric lyase, isocitritase, lyase, isocitrate, MtbIcl, PDP, petal death protein, SSO1333, threo-DS-Isocitrate glyoxylate-lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.1 isocitrate lyase

Cloned

Cloned on EC 4.1.3.1 - isocitrate lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a thioredoxin/His-tagged version expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli C41(DE3) cells
expressed in Escherichia coli RIL(DE3) cells
-
expressed in Escherichia coli strain BLR (DE3)
-
expressed in Escherichia coli strain JM 109
-
expressed in Escherichia coli Top10 cells
expressed in Saccharomyces cerevisiae
-
expression in Escherichia coli
expression in Saccharomyces cerevisiae
-
expression of the enzyme with a thrombin-cleavable N-terminal His6 tag in Escherichia coli strain BL21(DE3)
-
expresssion in Escherichia coli
from genomic DNA
-
from genomic DNA from strain H37Rv in pET21d for inducible expression in Escherichia coli C41
-
from genomic DNA into pQE30 for sequencing and subcloning into Escherichia coli-Mycobacterium shuttle plasmid pUV15(10) for expression as single protein of GFP fusion in Mycobacterium smegmatis also after infection of and phagocytosis by mouse macrophage cell line RAW264.7
-
from genomic DNA into pQE30 for sequencing and subcloning into Escherichia coli-Mycobacterium shuttle plasmid pUV15(10) for expression in Mycobacterium smegmatis also after infection of and phagocytosis by mouse macrophage cell line RAW264.7, amplified sequence carried non-sense mutation at position 681 (C to G)
-
gene aceA, phylogenetic analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene aceA, quantitative real-time PCR expression analysis, cloning in Escherichia coli strain JM109, overproduction of ICL by the Corynebacterium glutamicum DELTArneG mutant, which is deficient in RNase E/G, with prolonged half-life of the aceAmRNA
-
gene icl, expression of N-terminally His6-tagged enzyme in Escherichia coli strains XL-1 Blue, KM22 (a DELTArecBCD mutant), and ACA421, the latter is defective in isocitrate lyase
His-tagged version (without signal peptide (amino acid residue 1-12)) expressed in Escherichia coli BL21(DE3)
Icl and AceA are different open reading frames, both with isocitrate lyase activity
-
ICL loss-of-function mutant strain DELTAicl complemented with the functional ICL gene using the integrative vector pMV306
into vector pCR2.1-AfpyrG and overexpressed in the pyrA-strain AB4.1
-
lacZ fusion expressed Pseudomonas aeruginosa
-
pGSV3-lux ICL mutant
-
recombinant expression in Escherichia coli
-
wild-type and mutants cloned by the pET-3c-HIS/Escherichia coli BL21 expression system
-