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4.1.2.52: 4-hydroxy-2-oxoheptanedioate aldolase

This is an abbreviated version!
For detailed information about 4-hydroxy-2-oxoheptanedioate aldolase, go to the full flat file.

Reaction

4-hydroxy-2-oxoheptanedioate
=
pyruvate
+
succinate semialdehyde

Synonyms

4-hydroxy-2-oxo-heptane-1,7-dioate aldolase, HpaI, HpcH

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.52 4-hydroxy-2-oxoheptanedioate aldolase

Purification

Purification on EC 4.1.2.52 - 4-hydroxy-2-oxoheptanedioate aldolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by anion exchange, hydrophobic interaction, and gel filtration chormatography
metal affinity and size exclusion chromatography, Superdex 200 column used
-
wild type protein and R70A mutant protein purified to homogeneity using anion exchange, hydrophobic interaction, and gel filtration chormatography