4.1.2.52: 4-hydroxy-2-oxoheptanedioate aldolase

This is an abbreviated version!
For detailed information about 4-hydroxy-2-oxoheptanedioate aldolase, go to the full flat file.

4-hydroxy-2-oxo-heptane-1,7-dioate aldolase, HpaI, HpcH

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Results	in table
2354	AA Sequence
3	Cloned(Commentary)
4	Crystallization (Commentary)
2	General Information
1	General Stability
7	Inhibitors
18	kcat/KM [mM/s]
8	Ki Value [mM]
24	KM Value [mM]
12	Metals/Ions
3	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
5	Organism
6	Pathway
6	PDB ID
1	pH Range
8	Protein Variants
3	Purification (Commentary)
2	Reaction
5	Reference
19	Substrates and Products (Substrate)
2	Subunits
7	Synonyms
1	Systematic Name
19	Turnover Number [1/s]

Cloned on EC 4.1.2.52 - 4-hydroxy-2-oxoheptanedioate aldolase

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HpaI wild type and R70A mutant expressed in Escherichia coli BL21(lambda DE3) under the control of the T7 promoter from expression plasmid pT7-7

HpcH gene expressed in Escherichia coli strain B834 (DE3)

mutant HpaI enzymes expressed in Escherichia coli Bl21(lambda DE3) cells