4.1.2.52: 4-hydroxy-2-oxoheptanedioate aldolase
This is an abbreviated version!
For detailed information about 4-hydroxy-2-oxoheptanedioate aldolase, go to the full flat file.
Reaction
Synonyms
4-hydroxy-2-oxo-heptane-1,7-dioate aldolase, HpaI, HpcH
ECTree
4.1.2.52 4-hydroxy-2-oxoheptanedioate aldolaseAdvanced search results
results (
results (Engineering
Engineering on EC 4.1.2.52 - 4-hydroxy-2-oxoheptanedioate aldolase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D42A
inactive, the mutation leads to a concomitant loss of the metal ion
H45A
H45Q
R70A
H45A
by site-directed mutagenesis, mutant enzyme has 24fold higher dissociation constant for Co2+ compared to the wild-type enzyme, apparent Km value for Co2+ increases by about 800fold compared to the wild-type enzyme, different kcat and km values
H45A
the mutation leads to a decrease in kcat of the enzyme by 78fold
H45Q
by site-directed mutagenesis, mutant enzyme has 69fold higher dissociation constant for Co2+ compared to the wild-type enzyme, apparent Km value for Co2+ increases by about 800fold compared to the wild-type enzyme, different kcat and km values
H45Q
the mutation leads to a decrease in kcat of the enzyme by 2059fold
R70A
replacement by site-specific mutagenesis results in an enzyme that lacks both aldolase and decarboxylase activities. The mutant enzyme is also unable to catalyze pyruvate proton exchange
