Cloned (Comment) | Organism |
---|---|
HpaI wild type and R70A mutant expressed in Escherichia coli BL21(lambda DE3) under the control of the T7 promoter from expression plasmid pT7-7 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R70A | replacement by site-specific mutagenesis results in an enzyme that lacks both aldolase and decarboxylase activities. The mutant enzyme is also unable to catalyze pyruvate proton exchange | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyruvate | - |
Escherichia coli | |
sodium oxalate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
4-hydroxy-2-oxopentanoate | Km (app) with Co2+, pH 8.0, 25°C | Escherichia coli | |
0.0175 | - |
4-hydroxy-2-oxopentanoate | Km (app) with Mn2+, pH 8.0, 25°C | Escherichia coli | |
0.16 | - |
4-hydroxy-2-oxohexanoate | pH 8.0, 25°C | Escherichia coli | |
0.289 | - |
4-hydroxy-2-oxopentanoate | Km (app) with Mg2+, pH 8.0, 25°C | Escherichia coli | |
0.38 | - |
4-hydroxy-2-oxopentanoate | pH 8.0, 25°C | Escherichia coli | |
14.22 | - |
3-deoxy-D-manno-oct-2-ulosonic acid | i.e. KDO, pH 8.0, 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 0.5 mM chloride salt, 4% relative activity, with 4-hydroxy-2-oxopentanoate as substrate | Escherichia coli | |
Co2+ | 0.5 mM chloride salt, 100% relative activity, with 4-hydroxy-2-oxopentanoate as substrate | Escherichia coli | |
Cu2+ | 0.5 mM chloride salt, 1% relative activity, with 4-hydroxy-2-oxopentanoate as substrate | Escherichia coli | |
Fe2+ | 0.5 mM chloride salt, 57% relative activity, with 4-hydroxy-2-oxopentanoate as substrate | Escherichia coli | |
Mg2+ | 0.5 mM chloride salt, 49% relative activity, with 4-hydroxy-2-oxopentanoate as substrate | Escherichia coli | |
Mn2+ | 0.5 mM chloride salt, 99% relative activity, with 4-hydroxy-2-oxopentanoate as substrate | Escherichia coli | |
additional information | no activity with Ca2+, Fe3+, Cr3+ and Al3+ | Escherichia coli | |
Ni2+ | 0.5 mM chloride salt, 11% relative activity, with 4-hydroxy-2-oxopentanoate as substrate | Escherichia coli | |
Zn2+ | 0.5 mM chloride salt, 73% relative activity, with 4-hydroxy-2-oxopentanoate as substrate | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
subunit, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-2-oxoheptanedioate | Escherichia coli | - |
pyruvate + succinate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q47098 | - |
- |
Purification (Comment) | Organism |
---|---|
wild type protein and R70A mutant protein purified to homogeneity using anion exchange, hydrophobic interaction, and gel filtration chormatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxy-D-manno-oct-2-ulosonic acid | 3-deoxy-D-manno-oct-2-ulosonic acid i.e. KDO | Escherichia coli | ? | - |
? | |
4-hydroxy-2-oxoheptanedioate | - |
Escherichia coli | pyruvate + succinate semialdehyde | - |
? | |
4-hydroxy-2-oxohexanoate | - |
Escherichia coli | ? | - |
? | |
4-hydroxy-2-oxopentanoate | - |
Escherichia coli | ? | - |
? | |
additional information | the enzyme exhibits significant oxaloacetate decarboxylase activity, with a kcat value 2.4fold higher than the corresponding value for the aldol cleavage of 4-hydroxy-2-oxopentanoate | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HpaI | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
3-deoxy-D-manno-oct-2-ulosonic acid | i.e. KDO, pH 8.0, 25°C | Escherichia coli | |
229 | - |
4-hydroxy-2-oxohexanoate | pH 8.0, 25°C | Escherichia coli | |
353 | - |
4-hydroxy-2-oxopentanoate | pH 8.0, 25°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0055 | - |
sodium oxalate | pH 8.0, 25°C | Escherichia coli | |
0.53 | - |
pyruvate | pH 8.0, 25°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme is involved in the catabolic pathway of hydroxyphenylacetate | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03868 | - |
3-deoxy-D-manno-oct-2-ulosonic acid | i.e. KDO, pH 8.0, 25°C | Escherichia coli | |
940 | - |
4-hydroxy-2-oxopentanoate | pH 8.0, 25°C | Escherichia coli | |
1460 | - |
4-hydroxy-2-oxohexanoate | pH 8.0, 25°C | Escherichia coli |