4.1.2.25: dihydroneopterin aldolase

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For detailed information about dihydroneopterin aldolase, go to the full flat file.

Reaction

Synonyms

aldolase, dihydroneopterin, At3g11750, At5g62980, DHNA, DHNA-HPPK, dihydroneopterin aldolase, dihydroneopterin aldolase/epimerase, FASA, folA, FolB, FolB1, FolB2, MptD, Rv3607c

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.2 Aldehyde-lyases

                4.1.2.25 dihydroneopterin aldolase

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Results	in table
2	Activating Compound
26755	AA Sequence
2	Application
1	CAS Registry Number
17	Cloned(Commentary)
6	Crystallization (Commentary)
1	Expression
3	General Information
16	IC50 Value
28	Inhibitors
9	kcat/KM [mM/s]
35	KM Value [mM]
2	Metals/Ions
23	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
249	Organism
8	Pathway
42	PDB ID
5	pH Optimum
1	pH Stability
29	Protein Variants
18	Purification (Commentary)
5	Reaction
1	Reaction Type
44	Reference
2	Source Tissue
3	Specific Activity [micromol/min/mg]
78	Substrates and Products (Substrate)
27	Subunits
44	Synonyms
1	Systematic Name
2	Temperature Stability [°C]
25	Turnover Number [1/s]

Crystallization

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Crystallization on EC 4.1.2.25 - dihydroneopterin aldolase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

sitting-drop vapour-diffusion method by mixing 2 ml of a solution containing 11.5 mg of protein per ml with an equal volume of reservoir solution containing 0.1 M Tris hydrochloride (pH 7.2), 21% polyethylene glycol (PEG 2000 MME), and 115 mM cyclohexyl-butanoyl-N-hydroxyethylglucamide. Diffraction data are collected to a resolution of 2.2 A. The crystals belonged to space group P1 with the unit cell constants a = 63.5 A, b = 84.2 A, c = 89.1 A, alpha = 90.14°, beta = 89.9°, gamma = 76.2°, and has a solvent content of 42%

Arabidopsis thaliana

Q9SF23

666020

hanging drop vapor diffusion method, using 4.0 M sodium formate (pH 7.0)

Escherichia coli

P0AC16

747481

-

Staphylococcus aureus

-

5110

hanging drop vapour diffusion method, co-crystallization with monapterin or neopterin, in 1.4 M sodium acetate, 0.2 M imidazole, 0.1 M sodium cacodylate (pH 6.5), at 19°C

Staphylococcus aureus

P56740

681429

in complex with neopterin, an analog of dihydroneoptierin, and with monapterin, to 1.7 A and 1.68 A resolution, respectively. Active site residues E22, K100, and Y54 function coordinately during catalysis

Staphylococcus aureus

P56740

681429

hanging drop vapour diffusion method with 50 mM MOPS/NaOH (pH 7.0), 17.5% (w/v) methoxy-PEG 2000, 400 mM NaCl, 10 mM MgCl2, 2 mM dithiothreitol, 1 mM EDTA and 5% (v/v) glycerol

Streptococcus pneumoniae

-

675392