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Literature summary for 4.1.2.25 extracted from

  • Bauer, S.; Schott, A.K.; Illarionova, V.; Bacher, A.; Huber, R.; Fischer, M.
    Biosynthesis of tetrahydrofolate in plants: crystal structure of 7,8-dihydroneopterin aldolase from Arabidopsis thaliana reveals a novel adolase class (2004), J. Mol. Biol., 339, 967-979.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour-diffusion method by mixing 2 ml of a solution containing 11.5 mg of protein per ml with an equal volume of reservoir solution containing 0.1 M Tris hydrochloride (pH 7.2), 21% polyethylene glycol (PEG 2000 MME), and 115 mM cyclohexyl-butanoyl-N-hydroxyethylglucamide. Diffraction data are collected to a resolution of 2.2 A. The crystals belonged to space group P1 with the unit cell constants a = 63.5 A, b = 84.2 A, c = 89.1 A, alpha = 90.14°, beta = 89.9°, gamma = 76.2°, and has a solvent content of 42% Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SF23 recombinant
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Purification (Commentary)

Purification (Comment) Organism
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Arabidopsis thaliana