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Literature summary for 4.1.2.25 extracted from

  • Blaszczyk, J.; Li, Y.; Gan, J.; Yan, H.; Ji, X.
    Structural basis for the aldolase and epimerase activities of Staphylococcus aureus dihydroneopterin aldolase (2007), J. Mol. Biol., 368, 161-169.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, co-crystallization with monapterin or neopterin, in 1.4 M sodium acetate, 0.2 M imidazole, 0.1 M sodium cacodylate (pH 6.5), at 19°C Staphylococcus aureus
in complex with neopterin, an analog of dihydroneoptierin, and with monapterin, to 1.7 A and 1.68 A resolution, respectively. Active site residues E22, K100, and Y54 function coordinately during catalysis Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
monapterin
-
 Staphylococcus aureus
neopterin
-
 Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus P56740
-
-
Staphylococcus aureus P56740 bifunctional epimerase, EC 5.1.99.8, and aldolase
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography and Bio-Gel A 0.5 m gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydroneopterin
-
 Staphylococcus aureus 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
 ?
additional information enzyme catalyzes both the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin and the epimerization of 7,8-dihydroneopterin to 7,8-dihydromonapterin, reaction of EC 5.1.99.8 Staphylococcus aureus ?
-
 ?

Synonyms

Synonyms Comment Organism
DHNA
-
 Staphylococcus aureus
FolB
-
 Staphylococcus aureus