4.1.1.76: arylmalonate decarboxylase

This is an abbreviated version!
For detailed information about arylmalonate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.76

4.1.1.76

enantioselectivity

racemase

malonates

alcaligenes

bronchiseptica

racemisation

synthesis

bordetella

specifies

s-selective

succeeded

cofactor-free

profens

arylaliphatic

decarboxylases

biotechnology

Reaction

Synonyms

AMDase, aryl/alkenyl malonate decarboxylase, Arylmalonate decarboxylase, Arylmalonate decarboxylase (Alcaligenes bronchisepticus strain KU 1201), Decarboxylase, arylmalonate, Decarboxylase, arylmalonate (Bordetella bronchiseptica clone pAMD100 reduced), Decarboxylase, arylmalonate (Bordetella bronchiseptica strain KU1201 reduced)

ECTree

4 Lyases

4.1 Carbon-carbon lyases

4.1.1 Carboxy-lyases

4.1.1.76 arylmalonate decarboxylase

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Results	in table
285	AA Sequence
5	Application
2	CAS Registry Number
16	Cloned(Commentary)
2	Cofactor
3	Crystallization (Commentary)
11	Inhibitors
48	kcat/KM [mM/s]
1	Ki Value [mM]
98	KM Value [mM]
11	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
59	Organism
20	PDB ID
5	pH Optimum
4	pH Range
4	pH Stability
68	Protein Variants
17	Purification (Commentary)
2	Reaction
13	Reaction Type
45	Reference
9	Specific Activity [micromol/min/mg]
3	Storage Stability
158	Substrates and Products (Substrate)
6	Subunits
53	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
3	Temperature Range [°C]
7	Temperature Stability [°C]
96	Turnover Number [1/s]

Purification

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Purification on EC 4.1.1.76 - arylmalonate decarboxylase

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5 entries

DEAE-Sepharose column chromatography, DEAE-Toyopearl column chromatography, and Butyl-Toyopearl column chromatography

Achromobacter sp.

680955

DEAE-Toyopearl column chromatography and Butyl-Toyopearl column chromatography

Bordetella bronchiseptica

681486

diethylamino ethanol-Toyopearl column chromatography and Butyl-Toyopearl column chromatography

Bordetella bronchiseptica

677740

mutant enzymes Cys101Ser, Cys148Ser, Cys171Ser and Cys188Ser

Bordetella bronchiseptica

4832

Ni-NTA affinity chromatography

Bordetella bronchiseptica

Q05115

693635

Ni-Sepharose column chromatography, gel filtration

Bordetella bronchiseptica

Q05115

714745

recombinant AMDase

Bordetella bronchiseptica

650601, 650747, 650834

recombinant enzyme

Bordetella bronchiseptica

4831