4.1.1.76: arylmalonate decarboxylase

This is an abbreviated version!
For detailed information about arylmalonate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.76

4.1.1.76

enantioselectivity

racemase

malonates

alcaligenes

bronchiseptica

racemisation

synthesis

bordetella

specifies

s-selective

succeeded

cofactor-free

profens

arylaliphatic

decarboxylases

biotechnology

Reaction

Synonyms

AMDase, aryl/alkenyl malonate decarboxylase, Arylmalonate decarboxylase, Arylmalonate decarboxylase (Alcaligenes bronchisepticus strain KU 1201), Decarboxylase, arylmalonate, Decarboxylase, arylmalonate (Bordetella bronchiseptica clone pAMD100 reduced), Decarboxylase, arylmalonate (Bordetella bronchiseptica strain KU1201 reduced)

ECTree

4 Lyases

4.1 Carbon-carbon lyases

4.1.1 Carboxy-lyases

4.1.1.76 arylmalonate decarboxylase

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Results	in table
285	AA Sequence
5	Application
2	CAS Registry Number
16	Cloned(Commentary)
2	Cofactor
3	Crystallization (Commentary)
11	Inhibitors
48	kcat/KM [mM/s]
1	Ki Value [mM]
98	KM Value [mM]
11	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
59	Organism
20	PDB ID
5	pH Optimum
4	pH Range
4	pH Stability
68	Protein Variants
17	Purification (Commentary)
2	Reaction
13	Reaction Type
45	Reference
9	Specific Activity [micromol/min/mg]
3	Storage Stability
158	Substrates and Products (Substrate)
6	Subunits
53	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
3	Temperature Range [°C]
7	Temperature Stability [°C]
96	Turnover Number [1/s]

Cloned

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Cloned on EC 4.1.1.76 - arylmalonate decarboxylase

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cloned and overexpressed in Escherichia coli

Bordetella bronchiseptica

A0A0H3LJU9, Q05115

691765

complete gene is cloned into pBAD His A vector, and Escherichia coli Top10 cells are transformed

Bordetella bronchiseptica

Q05115

693635

expressed in Escherichia coli

3 entries

expressed in Escherichia coli JM109 cells

Bordetella bronchiseptica

679190

expressed in Escherichia coli strain JM109

2 entries

expressed in Escherichia coli Top10 cells

Bordetella bronchiseptica

Q05115

714745

expression in Escherichia coli

2 entries

mutant enzymes Cys101Ser, Cys148Ser, Cys171Ser and Cys188Ser expressed in Escherichia coli

Bordetella bronchiseptica

4832

overexpressed in Escherichia coli strain DH5alpha-MCR

Bordetella bronchiseptica

Q05115

690264