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Literature summary for 4.1.1.76 extracted from

  • Terao, Y.; Miyamoto, K.; Ohta, H.
    Introduction of single mutation changes arylmalonate decarboxylase to racemase (2006), Chem. Commun. (Camb. ), 34, 3600-3602.
    View publication on PubMed
Search for more literature for 4.1.1.76

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Bordetella bronchiseptica

Protein Variants

Protein Variants Comment Organism
G74C exhibits racemisation activity towards arylpropionates, in addition to its original decarboxylase activity Bordetella bronchiseptica
G74C/C188S gives the opposite enantiomer of arylpropionate compared to that obtained with the wild type enzyme Bordetella bronchiseptica

Organism

Organism UniProt Comment Textmining
Bordetella bronchiseptica
-
-
-
Bordetella bronchiseptica KU1201
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bordetella bronchiseptica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-methyl-alpha-(alpha-thienyl)malonate 350% activity compared to phenylmalonate, mutant enzyme G74C Bordetella bronchiseptica alpha-(alpha-thienyl)propionate + CO2
-
 ?
alpha-methyl-alpha-(alpha-thienyl)malonate 350% activity compared to phenylmalonate, mutant enzyme G74C Bordetella bronchiseptica KU1201 alpha-(alpha-thienyl)propionate + CO2
-
 ?
alpha-methyl-alpha-(beta-naphthyl)malonate 720% activity compared to phenylmalonate, mutant enzyme G74C Bordetella bronchiseptica alpha-(beta-naphthyl) propionate + CO2
-
 ?
alpha-methyl-alpha-(beta-naphthyl)malonate 720% activity compared to phenylmalonate, mutant enzyme G74C Bordetella bronchiseptica KU1201 alpha-(beta-naphthyl) propionate + CO2
-
 ?
alpha-phenylbutyrate 23% activity compared to phenylmalonate, mutant enzyme G74C Bordetella bronchiseptica ?
-
 ?
alpha-phenylbutyrate 23% activity compared to phenylmalonate, mutant enzyme G74C Bordetella bronchiseptica KU1201 ?
-
 ?
mandelate 14% activity compared to phenylmalonate, mutant enzyme G74C Bordetella bronchiseptica ?
-
 ?
additional information mutant enzyme G74C is unable to use iso-propyl, n-propyl, as well as carboxylic acid derivatives, such as alcohol, amide, nitrile, and esters as substrates Bordetella bronchiseptica ?
-
 ?
additional information mutant enzyme G74C is unable to use iso-propyl, n-propyl, as well as carboxylic acid derivatives, such as alcohol, amide, nitrile, and esters as substrates Bordetella bronchiseptica KU1201 ?
-
 ?
phenylglycine 5% activity compared to phenylmalonate, mutant enzyme G74C Bordetella bronchiseptica ?
-
 ?
Phenylmalonate 100% activity, mutant enzyme G74C Bordetella bronchiseptica Phenylacetate + CO2
-
 ?
Phenylmalonate 100% activity, mutant enzyme G74C Bordetella bronchiseptica KU1201 Phenylacetate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
AMDase
-
 Bordetella bronchiseptica