Literature summary for 4.1.1.76 extracted from

Kuettner, E.B.; Keim, A.; Kircher, M.; Rosmus, S.; Straeter, N.
Active-site mobility revealed by the crystal structure of arylmalonate decarboxylase from Bordetella bronchiseptica (2008), J. Mol. Biol., 377, 386-394.

Search for more literature for 4.1.1.76

Application

Application	Comment	Organism
biotechnology	enzyme is of biotechnological interest for its use in the synthesis of fine chemicals	Bordetella bronchiseptica

Cloned(Commentary)

Cloned (Comment)	Organism
complete gene is cloned into pBAD His A vector, and Escherichia coli Top10 cells are transformed	Bordetella bronchiseptica

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapour-diffusion hanging-drop setup	Bordetella bronchiseptica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25940	-	freshly purified AMDase	Bordetella bronchiseptica
25950	-	calculated molecular mass, including the C-terminal His-tag	Bordetella bronchiseptica
26030	-	crystals of AMDase can only be obtained after storage of the protein stock solution for about 4-5 weeks at 277 K. Electron spray ionization mass spectrometry measurement of a crystallizable AMDase sample shows two main components: 26025 (32%) and 26101 Da (68%), which are 83 and 159 Da higher than that of freshly purified AMDase (25942 Da)	Bordetella bronchiseptica
26100	-	crystals of AMDase can only be obtained after storage of the protein stock solution for about 4-5 weeks at 277 K. Electron spray ionization mass spectrometry measurement of a crystallizable AMDase sample shows two main components: 26025 (32%) and 26101 Da (68%), which are 83 and 159 Da higher than that of freshly purified AMDase (25942 Da)	Bordetella bronchiseptica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-aryl-2-methylmalonate	Bordetella bronchiseptica	-	2-arylpropionate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Bordetella bronchiseptica	Q05115	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA affinity chromatography	Bordetella bronchiseptica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-aryl-2-methylmalonate	-	Bordetella bronchiseptica	2-arylpropionate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
AMDase	-	Bordetella bronchiseptica
Arylmalonate decarboxylase	-	Bordetella bronchiseptica

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Release 2023.1 (January 2023)