4.1.1.76: arylmalonate decarboxylase

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For detailed information about arylmalonate decarboxylase, go to the full flat file.

Reaction

Synonyms

AMDase, aryl/alkenyl malonate decarboxylase, Arylmalonate decarboxylase, Arylmalonate decarboxylase (Alcaligenes bronchisepticus strain KU 1201), Decarboxylase, arylmalonate, Decarboxylase, arylmalonate (Bordetella bronchiseptica clone pAMD100 reduced), Decarboxylase, arylmalonate (Bordetella bronchiseptica strain KU1201 reduced)

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.1 Carboxy-lyases

                4.1.1.76 arylmalonate decarboxylase

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Results	in table
285	AA Sequence
5	Application
2	CAS Registry Number
16	Cloned(Commentary)
2	Cofactor
3	Crystallization (Commentary)
11	Inhibitors
48	kcat/KM [mM/s]
1	Ki Value [mM]
98	KM Value [mM]
11	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
59	Organism
20	PDB ID
5	pH Optimum
4	pH Range
4	pH Stability
68	Protein Variants
17	Purification (Commentary)
2	Reaction
13	Reaction Type
45	Reference
9	Specific Activity [micromol/min/mg]
3	Storage Stability
158	Substrates and Products (Substrate)
6	Subunits
53	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
3	Temperature Range [°C]
7	Temperature Stability [°C]
96	Turnover Number [1/s]

Crystallization

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Crystallization on EC 4.1.1.76 - arylmalonate decarboxylase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

G74C/C188S mutant in the liganded form at a resolution of 1.45 A, hanging drop vapor diffusion method, using 1.2 M ammonium sulfate, 15% (w/v) PEG5000, 10 mM bromophenylacetate, 1% (v/v) dioxane, and 70 mM HEPES (pH 7.6)

Bordetella bronchiseptica

Q05115

702364

hanging-drop vapour-diffusion method, crystallized with ammonium sulfate under alkaline pH conditions with the aim of understanding the mechanism of the enantioselective reaction

Bordetella bronchiseptica

Q05115

690264

vapour-diffusion hanging-drop setup

Bordetella bronchiseptica

Q05115

693635