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3.5.5.5: Arylacetonitrilase

This is an abbreviated version!
For detailed information about Arylacetonitrilase, go to the full flat file.

Word Map on EC 3.5.5.5

Reaction

4-Chlorophenylacetonitrile
+ 2 H2O =
4-chlorophenylacetate
 +
NH3

Synonyms

Arylacetonitrilase, arylacetonitrile-hydrolyzing nitrilase, arylacetonitrile-specific nitrilase, blr3397, enantioselective arylacetonitrilase, GPnor51, NIT, NitA, NitAd, NitAk2, NitAn, NitMp, NitP, nitrilase, nitrilase PpL19, Nitrilase, arylaceto-, NitTv

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.5 Arylacetonitrilase

Specific Activity

Specific Activity on EC 3.5.5.5 - Arylacetonitrilase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.44
pH 8.0, in supernatant of the recombinant Escherichia coli cells, activity is highest after 6 hours of cultivation
1.2
-
using 2-phenylpropionitrile as a substrate, at 30°C
1.26
pH 7.5, activity in enzyme preparation after purification, activity of the purified His-tagged protein was 28fold higher than the activity of the wild-type nitrilase
15.14
initial pH value 8.5
15.39
initial pH value 6.5
16.03
controlled pH value 6.5
16.27
controlled pH value 8.5
18.05
controlled pH value 7.5
18.92
initial pH value 7.5
21.7
Effect of IPG as inducer on the growth and enzyme production by Escherichia coli in a stirred tank reactor
22.8
Effect of lactose as inducer on the growth and enzyme production by Escherichia coli in a stirred tank reactor
3.26
-
35°C, pH 7.5
90
-
partially purified enzyme