3.5.5.5: Arylacetonitrilase

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For detailed information about Arylacetonitrilase, go to the full flat file.

Reaction

Synonyms

Arylacetonitrilase, arylacetonitrile-hydrolyzing nitrilase, arylacetonitrile-specific nitrilase, blr3397, enantioselective arylacetonitrilase, GPnor51, NIT, NitA, NitAd, NitAk2, NitAn, NitMp, NitP, nitrilase, nitrilase PpL19, Nitrilase, arylaceto-, NitTv

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.5 In nitriles

                3.5.5.5 Arylacetonitrilase

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Results	in table
8	Activating Compound
16	AA Sequence
9	Application
1	CAS Registry Number
20	Cloned(Commentary)
3	Expression
8	General Information
3	General Stability
61	Inhibitors
1	Ki Value [mM]
30	KM Value [mM]
5	Metals/Ions
23	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
5	Organic Solvent Stability
157	Organism
1	Oxidation Stability
23	pH Optimum
8	pH Range
17	pH Stability
80	Protein Variants
14	Purification (Commentary)
3	Reaction
4	Reaction Type
71	Reference
15	Specific Activity [micromol/min/mg]
8	Storage Stability
370	Substrates and Products (Substrate)
23	Subunits
44	Synonyms
1	Systematic Name
22	Temperature Optimum [°C]
6	Temperature Range [°C]
25	Temperature Stability [°C]
6	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.5.5.5 - Arylacetonitrilase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, in 10 mM potassium phosphate buffer, pH 7, 10 mM DTT and 50% glycerol, at least 5 months

Alcaligenes faecalis

-

32503

-20°C, pH 7.5, 14 days, 90% loss of activity, addition of 1 M ammonium sulfate almost completely stabilizes

Pseudomonas fluorescens

-

670163

-80°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, 20% glycerol, stable

Pseudomonas putida

-

667345

4°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, rapid loss of activity, presence of 20% glycerol stabilizes

Pseudomonas putida

-

667345

4°C, in 10 mM potassium phosphate buffer, pH 7, 10 mM DTT and 50% glycerol, at least 2 months, without glycerol 60% loss of activity within 1 week

Alcaligenes faecalis

-

32503

4°C, in the presence of 60% saturated ammonium sulfate, 2 M NaCl or 30% w/v propanediol, only about 5% loss of activity within 1 week

Alcaligenes faecalis

-

32503

5°C, in 0.02 M citrate buffer, pH 7-8, 10 days, about 50% loss of activity in 0.02 M Tris-HCl buffer, pH 9.3 within 10 days

Alcaligenes faecalis

-

32503

8°C, pH 7.5, 14 days, 75% loss of activity, addition of 1 M ammonium sulfate almost completely stabilizes

Pseudomonas fluorescens

-

670163