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3.5.5.5: Arylacetonitrilase

This is an abbreviated version!
For detailed information about Arylacetonitrilase, go to the full flat file.

Word Map on EC 3.5.5.5

Reaction

4-Chlorophenylacetonitrile
+ 2 H2O =
4-chlorophenylacetate
 +
NH3

Synonyms

Arylacetonitrilase, arylacetonitrile-hydrolyzing nitrilase, arylacetonitrile-specific nitrilase, blr3397, enantioselective arylacetonitrilase, GPnor51, NIT, NitA, NitAd, NitAk2, NitAn, NitMp, NitP, nitrilase, nitrilase PpL19, Nitrilase, arylaceto-, NitTv

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.5 Arylacetonitrilase

Cloned

Cloned on EC 3.5.5.5 - Arylacetonitrilase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparison, cloning and expression as His-tagged protein in Escherichia coli strains JM109 and BL21(DE3)
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli Rosetta2 (DE3) cells
-
expressed in Escherichia coli strain JM109
-
expressed in Pichia pastoris
-
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
-
expression in Escherichia coli JM 109, simultanuously with cassava (Manihot esculenta) oxynitrilase
expression in Escherichia coli strain BL21-Gold (DE3)
expression ion Escherichia coli
-
expression under the control of a rhamnose-inducible promoter in Escherichia coli strain JM109
-
overexpression in Escherichia coli BL21 (DE3) and formation ofd inclusion bodies that are active toward mandelonitrile and stable across a broad range of temperature and pH
-
recombinant functional expression of the enzyme in the cell envelope of Escherichia coli, where it is accessible for externally added protease
-
The nitrilase gene from Pseudomonas putida is cloned in plasmid pET 21b(+) and over-expressed as histidine-tagged protein in Escherichia coli strain BL21 (DE3)