3.5.5.5: Arylacetonitrilase

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For detailed information about Arylacetonitrilase, go to the full flat file.

Reaction

Synonyms

Arylacetonitrilase, arylacetonitrile-hydrolyzing nitrilase, arylacetonitrile-specific nitrilase, blr3397, enantioselective arylacetonitrilase, GPnor51, NIT, NitA, NitAd, NitAk2, NitAn, NitMp, NitP, nitrilase, nitrilase PpL19, Nitrilase, arylaceto-, NitTv

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.5 In nitriles

                3.5.5.5 Arylacetonitrilase

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Results	in table
8	Activating Compound
16	AA Sequence
9	Application
1	CAS Registry Number
20	Cloned(Commentary)
3	Expression
8	General Information
3	General Stability
61	Inhibitors
1	Ki Value [mM]
30	KM Value [mM]
5	Metals/Ions
23	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
5	Organic Solvent Stability
157	Organism
1	Oxidation Stability
23	pH Optimum
8	pH Range
17	pH Stability
80	Protein Variants
14	Purification (Commentary)
3	Reaction
4	Reaction Type
71	Reference
15	Specific Activity [micromol/min/mg]
8	Storage Stability
370	Substrates and Products (Substrate)
23	Subunits
44	Synonyms
1	Systematic Name
22	Temperature Optimum [°C]
6	Temperature Range [°C]
25	Temperature Stability [°C]
6	Turnover Number [1/s]

Activating Compound

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Activating Compound on EC 3.5.5.5 - Arylacetonitrilase

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2-mercaptoethanol

2 entries

dithiothreitol

Alcaligenes faecalis

-

activation

32502, 32503

phenylacetonitrile

Pseudomonas fluorescens

-

absolutely required as growth nitrogen source substrate for induction of the enzyme, cannot be substituted by ammonia or epsilon-caprolactam

652959

Sodium hydrosulfide

Alcaligenes faecalis

-

activation

32503

additional information

Exophiala oligosperma

-

expression is induced by growing the strain in the presence of different nitriles and /or complex or inorganic nitrogen sources. The highest nitrile hydrolysing activity is observed with cells grown with 2-cyanopyridine and NaNO3

701254

-

2-mercaptoethanol

Alcaligenes faecalis

-

activation

32502, 32503

2-mercaptoethanol

Pseudomonas fluorescens

-

1.4fold at 50 mM, 1.6fold activation at 100 mM

652959