3.5.5.5: Arylacetonitrilase

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For detailed information about Arylacetonitrilase, go to the full flat file.

Word Map on EC 3.5.5.5

3.5.5.5

mandelonitrile

enantioselectivity

fluorescens

arylacetonitriles

enantiomeric

s-mandelic

phenylacetonitrile

alcaligenes

r,s-mandelonitrile

benzaldehyde

synthesis

arylacetic

esculenta

enantiospecificity

ultrafast

psychrotolerans

s-selectivity

manihot

biotechnology

Reaction

+ 2 H2O =

Synonyms

Arylacetonitrilase, arylacetonitrile-hydrolyzing nitrilase, arylacetonitrile-specific nitrilase, blr3397, enantioselective arylacetonitrilase, GPnor51, NIT, NitA, NitAd, NitAk2, NitAn, NitMp, NitP, nitrilase, nitrilase PpL19, Nitrilase, arylaceto-, NitTv

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.5 In nitriles

3.5.5.5 Arylacetonitrilase

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Results	in table
8	Activating Compound
16	AA Sequence
9	Application
1	CAS Registry Number
20	Cloned(Commentary)
3	Expression
8	General Information
3	General Stability
61	Inhibitors
1	Ki Value [mM]
30	KM Value [mM]
5	Metals/Ions
23	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
5	Organic Solvent Stability
157	Organism
1	Oxidation Stability
23	pH Optimum
8	pH Range
17	pH Stability
80	Protein Variants
14	Purification (Commentary)
3	Reaction
4	Reaction Type
71	Reference
15	Specific Activity [micromol/min/mg]
8	Storage Stability
370	Substrates and Products (Substrate)
23	Subunits
44	Synonyms
1	Systematic Name
22	Temperature Optimum [°C]
6	Temperature Range [°C]
25	Temperature Stability [°C]
6	Turnover Number [1/s]

pH Range

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pH Range on EC 3.5.5.5 - Arylacetonitrilase

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3 - 7

Pseudomonas fluorescens

684655

4.5

2 entries

5 - 9

Bradyrhizobium japonicum

687882

6.4 - 9.4

Alcaligenes faecalis

about half-maximal activity at pH 6.4 and 9.4, 2-thiopheneacetonitrile as substrate

32503

6.5 - 10

Pseudomonas psychrotolerans

pH 6.5: about 65% of maximal activity, pH 10.0: about 70% of maximal activity

756265