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3.5.5.5: Arylacetonitrilase

This is an abbreviated version!
For detailed information about Arylacetonitrilase, go to the full flat file.

Word Map on EC 3.5.5.5

Reaction

4-Chlorophenylacetonitrile
+ 2 H2O =
4-chlorophenylacetate
 +
NH3

Synonyms

Arylacetonitrilase, arylacetonitrile-hydrolyzing nitrilase, arylacetonitrile-specific nitrilase, blr3397, enantioselective arylacetonitrilase, GPnor51, NIT, NitA, NitAd, NitAk2, NitAn, NitMp, NitP, nitrilase, nitrilase PpL19, Nitrilase, arylaceto-, NitTv

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.5 Arylacetonitrilase

KM Value

KM Value on EC 3.5.5.5 - Arylacetonitrilase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.015
2-Chlorobenzylcyanide
-
-
0.38
2-chloromandelonitrile
-
at pH 7.0 and 30°C
3.4 - 11.4
2-hydroxy-2-phenylacetonitrile
0.091
2-Thiopheneacetonitrile
-
-
0.065
3-Chlorobenzylcyanide
-
-
0.009
3-Indoleacetonitrile
-
-
0.016
3-Thiopheneacetonitrile
-
-
0.023
3-tolylacetonitrile
-
-
0.022
4-Aminobenzylcyanide
-
-
0.333
4-Chlorobenzylcyanide
-
-
0.13
4-Fluorobenzylcyanide
-
-
20
4-Hydroxyphenylacetonitrile
-
pH 7.0, 30°C
0.105
4-Nitrobenzylcyanide
-
-
0.123
4-tolylacetonitrile
-
-
0.66
acrylonitrile
-
-
0.01
benzylcyanide
-
-
5.31
indole 3-acetonitrile
-
35°C, pH 7.5
4.74 - 13.4
mandelonitrile
3.61
phenyl acetonitrile
-
35°C, pH 7.5
0.065 - 4.36
phenylacetonitrile
0.8 - 1.3
phenylpropionitrile
0.087
rac 2-(methoxy)-mandelonitrile
-
pH 7.4, 30°C
additional information
additional information
-
kinetic and chiral analysis, overview
-