Literature summary for 3.5.5.5 extracted from

Kiziak, C.; Klein, J.; Stolz, A.
Influence of different carboxy-terminal mutations on the substrate-, reaction- and enantiospecificity of the arylacetonitrilase from Pseudomonas fluorescens EBC191 (2007), Protein Eng. Des. Sel., 20, 385-396.

Search for more literature for 3.5.5.5

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain JM109	Pseudomonas fluorescens

Protein Variants

Protein Variants	Comment	Organism
additional information	the C-terminally truncated nitrilase variants demonstrate that deletions up to about 30 amino acids do not significantly change the relevant characteristics of the enzyme, in contrast, longer C-terminal deletions of 47-67 amino acids result in significant decreases in enzyme activities and only about 10% of the activity is found compared with the wild type enzyme, deletion mutants Nit(del-C-20) and Nit(del-C-32) strongly resemble the wild type enzyme, mutants Nit(del-C-47) to Nit(del-C-67) demonstrate reduced nitrilase activities against 2-phenylpropionitrile and show a significant decreased stability of the nitrilase activity compared with the wild type enzyme, deletion mutants also show an increased formation of amide in relation to the acid formation (up to almost 10% compared to 0.2% with the wild type enzyme)	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	-	-
Pseudomonas fluorescens EBC191	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.2	-	using 2-phenylpropionitrile as a substrate, at 30°C	Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phenylpropionitrile + 2 H2O	-	Pseudomonas fluorescens	2-phenylpropionic acid + NH3	(R)-2-phenylpropionic acid is preferentially formed	?
2-phenylpropionitrile + 2 H2O	-	Pseudomonas fluorescens EBC191	2-phenylpropionic acid + NH3	(R)-2-phenylpropionic acid is preferentially formed	?
mandelonitrile + 2 H2O	-	Pseudomonas fluorescens	mandelic acid + NH3	slight preference for the formation of (R)-mandelic acid	?
mandelonitrile + 2 H2O	-	Pseudomonas fluorescens EBC191	mandelic acid + NH3	slight preference for the formation of (R)-mandelic acid	?

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Release 2023.1 (January 2023)