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3.5.4.6: AMP deaminase

This is an abbreviated version!
For detailed information about AMP deaminase, go to the full flat file.

Word Map on EC 3.5.4.6

Reaction

AMP
+
H2O
=
IMP
+
NH3

Synonyms

5'-adenosine monophosphate deaminase, 5'-adenylic acid deaminase, 5-adenylate deaminase, 5-adenylic acid deaminase, 5-AMP aminohydrolase, 5-AMP deaminase, adenosine 5-monophosphate deaminase, adenosine 5-phosphate aminohydrolase, adenosine 5-phosphate deaminase, adenosine monophosphate deaminase, adenosine monophosphate deaminase 3, adenosine monophosphate deaminase-1, adenosine-5'-monophosphate deaminase, adenyl deaminase, adenylate aminohydrolase, adenylate deaminase, adenylate desaminase, adenylic acid deaminase, adenylic deaminase, AMP aminase, AMP deaminase, AMP deaminase 3, AMP deaminase H-type, AMP deaminase isoform E, AMP deaminase type 3, AMP deaminase1, AMP-aminohydrolase, AMP-deaminase, AmpD, AMPD1, AMPD2, AMPD3, AMPDA, deaminase, adenylate, embryonic factor 1, EMBRYONIC FACTOR1, Erythrocyte AMP deaminase, FAC1, Heart-type AMPD, muscle AMP deaminase, Myoadenylate deaminase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.6 AMP deaminase

Molecular Weight

Molecular Weight on EC 3.5.4.6 - AMP deaminase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
140000 - 400000
-
3 different oligomeric enzyme forms of 140 kDa, 280 kDa, and 400 kDa, gel filtration
145000
-
gel filtration
150000
-
gel filtration
160000
-
myosin-bound enzyme, SDS-PAGE
276000
-
sucrose density gradient centrifugation
285000
-
sucrose density gradient centrifugation
320000
-
calculation from sedimentation constant and diffusion coefficient
330000
-
gel filtration
37000
-
x * 37000, SDS-PAGE
55000
60000
-
the SDS-PAGE shows that the enzyme is not electrophoretically homogenous, with two bands present at a molecular mass between about 55000 and 60000 Da
68000
69000
-
4 * 69000, SDS-PAGE
70000
-
2 * 85000 (catalytic subunits) + 2 * 70000 (histidine-proline-rich-glycoprotein subunits)
75000
-
gel filtration
80000
-
4 * 80000, stabilized by phosphate
83000
-
4 * 83000, SDS-PAGE
85000
85390
-
SDS-PAGE
92000
-
SDS-PAGE, 92 and 68 kDa fragments of the enzyme react with specificpolyclonal anti-(human) AMPD2 antibodies