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3.5.4.6: AMP deaminase

This is an abbreviated version!
For detailed information about AMP deaminase, go to the full flat file.

Word Map on EC 3.5.4.6

Reaction

AMP
+
H2O
=
IMP
 +
NH3

Synonyms

5'-adenosine monophosphate deaminase, 5'-adenylic acid deaminase, 5-adenylate deaminase, 5-adenylic acid deaminase, 5-AMP aminohydrolase, 5-AMP deaminase, adenosine 5-monophosphate deaminase, adenosine 5-phosphate aminohydrolase, adenosine 5-phosphate deaminase, adenosine monophosphate deaminase, adenosine monophosphate deaminase 3, adenosine monophosphate deaminase-1, adenosine-5'-monophosphate deaminase, adenyl deaminase, adenylate aminohydrolase, adenylate deaminase, adenylate desaminase, adenylic acid deaminase, adenylic deaminase, AMP aminase, AMP deaminase, AMP deaminase 3, AMP deaminase H-type, AMP deaminase isoform E, AMP deaminase type 3, AMP deaminase1, AMP-aminohydrolase, AMP-deaminase, AmpD, AMPD1, AMPD2, AMPD3, AMPDA, deaminase, adenylate, embryonic factor 1, EMBRYONIC FACTOR1, Erythrocyte AMP deaminase, FAC1, Heart-type AMPD, muscle AMP deaminase, Myoadenylate deaminase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.6 AMP deaminase

General Stability

General Stability on EC 3.5.4.6 - AMP deaminase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
an apparent 10000 Da reduction in molecular mass of the native 85000 Da enzyme subunit with its conversion to a 75000 Da core is observed on storage of AMPD
-
ATP, ADP, GTP and alkali metal ions protect against heat inactivation
-
EDTA stabilizes the purified enzyme during storage
-
enzyme is associated to the histidine-proline-rich-glycoprotein HPRG via its catalytic subunit in a protein-protein complex, which is critical for the enzyme stability
-
freezing, unstable
-
heating in the presence of low KCl (150 mM) results in a rapid loss in activity with only 25% of initial activity being left after 30 min, about half of the initial activity remains after heating for 30 min in 25 mM histidine solution, solution, incubation in imidazole or phosphate solutions results in greater stability over 30 min of heating with AMPD activity decreasing by about 20%
-
phosphate stabilizes the tetrameric structure of the enzyme
-
repeated freezing and thawing, stable
-
sulfhydryl reagents and monovalent cations in high concentrations stabilize
-
additional information
-