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Literature summary for 3.5.4.6 extracted from

  • Lushchak, V.I.; Husak, V.V.; Storey, K.B.
    Regulation of AMP-deaminase activity from white muscle of common carp Cyprinus carpio (2008), Comp. Biochem. Physiol. B, 149B, 362-369.
No PubMed abstract available
Search for more literature for 3.5.4.6

Activating Compound

Activating Compound Comment Organism Structure
ADP 6.1fold activation at 2 mM Cyprinus carpio
ATP 3.7fold activation at 2 mM Cyprinus carpio

General Stability

General Stability Organism
heating in the presence of low KCl (150 mM) results in a rapid loss in activity with only 25% of initial activity being left after 30 min, about half of the initial activity remains after heating for 30 min in 25 mM histidine solution, solution, incubation in imidazole or phosphate solutions results in greater stability over 30 min of heating with AMPD activity decreasing by about 20% Cyprinus carpio

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ at 10 mM Ca2+ inhibition of AMPD is 28% Cyprinus carpio
fluoride 8 mM fluoride reduce AMPD activity by 54% Cyprinus carpio
H2O2 treatment with 0.1 mM H2O2 reduces activity by half after about 50 min, 50% of activity is lost in about 25 min when AMPD is incubated with both iron and H2O2 Cyprinus carpio
K+ inhibitory at concentrations above 300 mM, at 500 mM 62% of initial activities is left Cyprinus carpio
Mg2+ at 10 mM Mg2+ inhibition of AMPD is 13% Cyprinus carpio
additional information incubation with 0.005 mM FeSO4 does not affect AMPD activity Cyprinus carpio
Na+ inhibitory at concentrations above 250 mM, at 500 mM 46% of initial activities is left Cyprinus carpio
phosphate 10 mM phosphate lower activity by 39% Cyprinus carpio

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates at low concentrations of 100-150 mM, in the presence of 100 mM K+ enzyme activity is increased by 1.7fold Cyprinus carpio
Na+ activates at low concentrations of 100-150 mM, in the presence of 100 mM Na+ enzyme activity is increased by 2.4fold Cyprinus carpio

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 subunit, SDS-PAGE Cyprinus carpio

Organism

Organism UniProt Comment Textmining
Cyprinus carpio
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phosphocellulose column chromatography Cyprinus carpio

Source Tissue

Source Tissue Comment Organism Textmining
gill
-
 Cyprinus carpio
-
kidney
-
 Cyprinus carpio
-
skeletal muscle higher activity in white muscle compared to red muscle Cyprinus carpio
-

Storage Stability

Storage Stability Organism
2-4°C, 1 M KCl, at least 1 month, no loss of activity Cyprinus carpio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + H2O
-
 Cyprinus carpio IMP + NH3
-
 ?

Synonyms

Synonyms Comment Organism
AMP-aminohydrolase
-
 Cyprinus carpio
AMP-deaminase
-
 Cyprinus carpio
AmpD
-
 Cyprinus carpio

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 heating at 50°C quickly destroys AMPD activity when the enzyme is incubated in distilled water, AMPD remains fully active over 30 min in the presence 1 M KCl at 50°C Cyprinus carpio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
 Cyprinus carpio

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7.5 slowly reduces its activity on the both sides of the optimum pH 6.3, at pH values of 5.5, 7.0 and 7.5, AMPD shows 13, 31 and 65% lower activity, respectively Cyprinus carpio