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3.5.3.1: arginase

This is an abbreviated version!
For detailed information about arginase, go to the full flat file.

Word Map on EC 3.5.3.1

Reaction

L-arginine
+
H2O
=
L-ornithine
 +
Urea

Synonyms

ARG, Arg I, Arg II, ARG1, ARG2, ARGAH1, ARGAH2, ArgI, arginase, arginase 1, arginase 1a, arginase 1b, arginase 2a, arginase 2b, arginase I, arginase II, arginase type I, arginase-1, arginase-2, arginase1, arginine amidinase, arginine amidohydrolase-1, arginine amidohydrolase-2, arginine transamidinase, canavanase, human arginase type I, Kidney-type arginase, L-arginase, L-arginine amidino hydrolase, L-arginine amidinohydrolase, L-arginine amidohydrolase, L-arginine ureahydrolase, Liver-type arginase, mitochondrial arginase, More, Non-hepatic arginase, PMN arginase, recombinant human arginase I, rhArg-PEG, rhArg1, RocF, serum arginase, SmARG, tissue arginase, type II arginase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.1 arginase

Purification

Purification on EC 3.5.3.1 - arginase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2857 purification fold and a 20% recovery
-
ammonium sulfate precipitation, AH-Sepharose 4B column chromatography, and gel filtration
ammonium sulfate precipitation, DEAE column chromatography, arginine-Sepharose column chromatography, and Sephadex G-100 gel filtration
ammonium sulfate precipitation, DEAE-Sepharose column chromatography and gel filtration
-
cobalt-nitrilotriacetic acid column chromatography, and Q-HP anion-exchange column chromatography
DEAE-cellulose column chromatography
-
DEAE-cellulose column chromatography, CM-Sephadex column chromatography, and Bio-Gel P-100 gel filtration
-
expressed in Escherichia coli
HiTrap Ni2+-chelating Sepharose column chromatography
-
Ni2+-resin column chromatography
nickel affinity column chromatography
-
partial
partially from vaginal tissue
-
recombinant enzyme from Escherichia coli
-
recombinant enzyme to homogeneity
-
recombinant wild-type and mutant enzymes from Escherichia coli
recombinant wild-type and truncated mutant enzymes from Escherichia coli BL21(DE3)
-
two isoenzymes
-