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Literature summary for 3.5.3.1 extracted from
- Characterization of Bacillus anthracis arginase: effects of pH, temperature, and cell viability on metal preference (2008), BMC Biochem., 9, 15.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Bacillus anthracis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | displays remarkable activity in the absence of exogenous metals, although manganese, cobalt, and nickel all improve activity. Enzyme shifts its metal preference from Ni, Co,and Mn (decreasing order) when assayed at pH 6 to Ni, Mn, and Co (decreasing order) at pH 9 | Bacillus anthracis |  |
| Co2+ | enzyme is optimally active with cobalt at pH 6 | Helicobacter pylori | |
| Mn2+ | displays remarkable activity in the absence of exogenous metals, although manganese, cobalt, and nickel all improve activity. Enzyme shifts its metal preference from Ni, Co,and Mn (decreasing order) when assayed at pH 6 to Ni, Mn, and Co (decreasing order) at pH 9 | Bacillus anthracis | |
| additional information | Fe2+, Ca2+, Mg2+, Zn2+, Cu2+ have no effect on enzyme activity | Bacillus anthracis | |
| Ni2+ | optimal arginase activity occurrs with nickel at an alkaline pH | Bacillus anthracis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | - |
7702 | - |
| Bacillus anthracis 7702 | - |
7702 | - |
| Helicobacter pylori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus anthracis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
arginase is up to 1000times more active than Helicobacter pylori arginase | Bacillus anthracis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | - |
Helicobacter pylori | L-ornithine + urea | - |
? | |
| L-arginine + H2O | - |
Bacillus anthracis | L-ornithine + urea | - |
? | |
| L-arginine + H2O | - |
Bacillus anthracis 7702 | L-ornithine + urea | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arginase | - |
Helicobacter pylori |
| arginase | - |
Bacillus anthracis |
| RocF | - |
Helicobacter pylori |
| RocF | - |
Bacillus anthracis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | activity increased about 10fold following treatment with heat (50-55°C, 30 min) at pH 9.0 in the absence of metal versus no heat, in the presence of various metals, heat treatment does not affect arginase activity at either pH 6.0 or 9.0, when compared to samples that are not heat-treated | Bacillus anthracis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Helicobacter pylori |
| 6 | - |
Escherichia coli (pBS-barocF) has optimal arginase activity at both pH 6.0 and 9.0 with nickel, with lower activity in the presence of cobalt or manganese | Bacillus anthracis |
| 9 | - |
Escherichia coli (pBS-barocF) has optimal arginase activity at both pH 6.0 and 9.0 with nickel, with lower activity in the presence of cobalt or manganese | Bacillus anthracis |
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