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Literature summary for 3.5.3.1 extracted from

  • D'Antonio, E.L.; Ullman, B.; Roberts, S.C.; Dixit, U.G.; Wilson, M.E.; Hai, Y.; Christianson, D.W.
    Crystal structure of arginase from Leishmania mexicana and implications for the inhibition of polyamine biosynthesis in parasitic infections (2013), Arch. Biochem. Biophys., 535, 163-176.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leishmania mexicana

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with L-ornithine and inhibitors, hanging drop vapor diffusion method, using 10% (w/v) polyethylene glycol 10000, 5% (v/v) (+/-)-2-methyl-2,4-pentanediol, and 0.1 M HEPES, pH 7.3 Leishmania mexicana

Inhibitors

Inhibitors Comment Organism Structure
2(S)-amino-6-boronohexanoic acid
-
 Leishmania mexicana
nor-Nomega-hydroxy-L-arginine
-
 Leishmania mexicana
S-(2-boronoethyl)-L-cysteine
-
 Leishmania mexicana

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
 Leishmania mexicana 20015
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ contains Mn2+ Leishmania mexicana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + H2O Leishmania mexicana
-
 L-ornithine + urea
-
 ?
L-arginine + H2O Leishmania mexicana MNYC/BZ/62/M379
-
 L-ornithine + urea
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania mexicana Q6TUJ5
-
-
Leishmania mexicana MNYC/BZ/62/M379 Q6TUJ5
-
-

Purification (Commentary)

Purification (Comment) Organism
cobalt-nitrilotriacetic acid column chromatography, and Q-HP anion-exchange column chromatography Leishmania mexicana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
 Leishmania mexicana L-ornithine + urea
-
 ?
L-arginine + H2O
-
 Leishmania mexicana MNYC/BZ/62/M379 L-ornithine + urea
-
 ?

Subunits

Subunits Comment Organism
trimer
-
 Leishmania mexicana

Synonyms

Synonyms Comment Organism
ARG
-
 Leishmania mexicana

General Information

General Information Comment Organism
physiological function the enzyme catalyzes the first committed step in the biosynthesis of polyamines that enable cell growth and survival Leishmania mexicana