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Literature summary for 3.5.3.1 extracted from
- Unusual hepatic mitochondrial arginase in an Indian air-breathing teleost, Heteropneustes fossilis: purification and characterization (2013), Comp. Biochem. Physiol. B, 164, 133-141.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 20.41% residual activity at 1 mM; 20.41% residual activity at 1 mM | Heteropneustes fossilis |  |
| Cd2+ | 58.78% residual activity at 1 mM; 58.78% residual activity at 1 mM | Heteropneustes fossilis | |
| Cu2+ | 30.2% residual activity at 1 mM; 30.2% residual activity at 1 mM | Heteropneustes fossilis | |
| Fe2+ | 77.14% residual activity at 1 mM; 77.14% residual activity at 1 mM | Heteropneustes fossilis | |
| Fe3+ | 77.14% residual activity at 1 mM; 77.14% residual activity at 1 mM | Heteropneustes fossilis | |
| L-ornithine | mixed type inhibition; mixed type inhibition | Heteropneustes fossilis | |
| Nomega-hydroxy-L-arginine | mixed type inhibition; mixed type inhibition | Heteropneustes fossilis | |
| Zn2+ | 33.47% residual activity at 1 mM; 33.47% residual activity at 1 mM | Heteropneustes fossilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.25 | - |
L-arginine | at pH 7.0 and 30°C | Heteropneustes fossilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Heteropneustes fossilis | 5829 | - |
| mitochondrion | - |
Heteropneustes fossilis | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | effective activator, 144% activity at 1 mM | Heteropneustes fossilis | |
| Mn2+ | effective activator, 207% activity at 1 mM | Heteropneustes fossilis | |
| additional information | Ca2+, Na+, K+, Mg2+, and Ni2+ do not show any significant effect on enzyme activity | Heteropneustes fossilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
2 * 48000, SDS-PAGE | Heteropneustes fossilis |
| 96000 | - |
gel filtration | Heteropneustes fossilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | Heteropneustes fossilis | - |
L-ornithine + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Heteropneustes fossilis | A0A0D5ZYA4 | - |
- |
| Heteropneustes fossilis | U6BQJ3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE column chromatography, arginine-Sepharose column chromatography, and Sephadex G-100 gel filtration | Heteropneustes fossilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Heteropneustes fossilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.184 | - |
mitochondrial extract, at pH 7.0 and 30°C | Heteropneustes fossilis |
| 0.694 | - |
after 150.41fold purification, at pH 7.0 and 30°C | Heteropneustes fossilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | - |
Heteropneustes fossilis | L-ornithine + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 48000, SDS-PAGE | Heteropneustes fossilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Arg I | - |
Heteropneustes fossilis |
| Arg II | - |
Heteropneustes fossilis |
| arginase I | - |
Heteropneustes fossilis |
| arginase II | - |
Heteropneustes fossilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Heteropneustes fossilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
purified hepatic mitochondrial arginase II is stable at 55°C | Heteropneustes fossilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
- |
Heteropneustes fossilis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
Nomega-hydroxy-L-arginine | at pH 7.0 and 30°C | Heteropneustes fossilis | |
| 2.16 | - |
L-ornithine | at pH 7.0 and 30°C | Heteropneustes fossilis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Heteropneustes fossilis | isoelectric focusing | - |
7.5 |
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