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3.5.1.98: histone deacetylase

This is an abbreviated version!
For detailed information about histone deacetylase, go to the full flat file.

Word Map on EC 3.5.1.98

Reaction

hydrolysis of an N6-acetyl-lysine residue of a histone to yield a deacetylated histone =

Synonyms

acetyl-lysine deacetylase, AhHDA1, AtHD1, class I acetyl-lysine deacetylase, class I HDAC, class I histone deacetylase, class II Hda1 HDAC, class II histone deacetylase, class IIa HDAC, class IIa histone deacetylase, Clr6, cytoplasmic deacetylase, deacetylase-like amidohydrolase, dRPD3, FB188 HDAH, Glyma.01 g245100, Glyma.04 g000200, Glyma.04 g187000, Glyma.04 g187100, Glyma.05 g012900, Glyma.05 g021400, Glyma.05 g040600, Glyma.05 g192600, Glyma.06 g000100, Glyma.06 g178700, Glyma.11 g000300, Glyma.11 g187800, Glyma.12 g086700, Glyma.12 g188200, Glyma.17 g078000, Glyma.17 g085700, Glyma.17 g120900, Glyma.17 g229600, GmHDA1, GmHDA10, GmHDA11, GmHDA12, GmHDA13, GmHDA14, GmHDA15, GmHDA16, GmHDA17, GmHDA18, GmHDA2, GmHDA3, GmHDA4, GmHDA5, GmHDA6, GmHDA7, GmHDA8, GmHDA9, GmHDAC, HD1B, HD2, HD8, HDA, HDA-1, HDA-2, HDA-3, HDA-4, hda1, HDA15, HDA19, HDA9, HdaA, HDAC, HDAC 11, HDAC 3, HDAC1, HDAC10, HDAC2, HDAC2-1, HDAC2-2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC6p114, HDAC6p131, HDAC7, HDAC8, HDAC9, HDAH, HDLP, HDT1, HDT2, histone deacetylase, histone deacetylase 1, histone deacetylase 10, histone deacetylase 11, histone deacetylase 2, histone deacetylase 3, histone deacetylase 4, histone deacetylase 6, histone deacetylase 7, histone deacetylase 8, histone deacetylase-1, histone deacetylase-7, histone deacetylase-like amidohydrolase, histone deacetylase-like protein, histone deacetylase1, Hos2, HosB, PA3774, pfHDAC-1, RPD3, Set3 complex, Set3C, tubulin deacetylase, zinc-dependent histone deacetylase, Zn(II)-dependent deacetylase, Zn-HDAC

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.98 histone deacetylase

Purification

Purification on EC 3.5.1.98 - histone deacetylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by affinity chromatography on a 5-ml HiTrap Chelating column charged with Ni2+ and by anion exchange chromatography on a Source 30Q column
cytoplasmic lysates are prepared
enzyme copurifies as a complex with a protein related to the retinoblastoma-associated protein, Rbap46
partially by isolation of nuclei from Arachis hygogaea leaves, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
proteins are expressed in Escherichia coli BL21DE3 cells and purified using nickel nitrilotriacetic acid or TALON affinity resin, a GE HiTrap Q HP and a Superdex 26-60 column
recombinant enzyme purified aerobically from Escherichia coli contains 8fold more iron than zinc before dialysis
recombinant HDAC1 from Escherichia coli strain TOP10 by anion exchange chromatography, ultrafiltration, heparin affinity chromatography, poly-L-lysine affinity chromatography, and gel filtration. The effects of chaotropic species, weak kosmotropes, amino acids, reducing or denaturing agents, detergents, alcohols, and saturated fatty acids are tested to dissociate the protein aggregates, overview
recombinant His-tagged wild-type and mutant ARB2 domains from Escherichia coli strain BL21(DE3) by nickel affinity chroatography and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant N-terminally His6-atgegd enzyme from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography and dialysis
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recombinant protein
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using a mono Q HR5-5 column
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using an engineered HPC4 epitope tag at the C-terminus
via affinity chromatography using irreversible inhibitor trapoxin
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