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Literature summary for 3.5.1.98 extracted from

  • Kim, B.; Pithadia, A.S.; Fierke, C.A.
    Kinetics and thermodynamics of metal-binding to histone deacetylase 8 (2015), Protein Sci., 24, 354-365.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-(3,6-dihydroxy-9H-xanthen-9-yl)-5-(8-(hydroxyamino)-8-oxooctanamido)benzoic acid synthesis, overview Homo sapiens
2-(3,6-dihydroxy-9H-xanthen-9-yl)-5-(8-methoxy-8-oxooctanamido)benzoic acid synthesis, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ isozyme HDAC8 has higher activity with a bound Fe(II) than Zn(II), although Fe(II)-HDAC8 rapidly loses activity under aerobic conditions Homo sapiens
Zn2+ Zn(II)-dependent deacetylase, but isozyme HDAC8 has higher activity with a bound Fe(II) than Zn(II), although Fe(II)-HDAC8 rapidly loses activity under aerobic conditions Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BY41
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Homo sapiens

Synonyms

Synonyms Comment Organism
HDAC8
-
Homo sapiens
histone deacetylase 8
-
Homo sapiens
Zn(II)-dependent deacetylase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens