3.5.1.14: N-acyl-aliphatic-L-amino acid amidohydrolase
This is an abbreviated version!
For detailed information about N-acyl-aliphatic-L-amino acid amidohydrolase, go to the full flat file.
Word Map on EC 3.5.1.14
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3.5.1.14
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n-acetylated
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synthesis
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n-acetyl-l-methionine
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mercapturic
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acylases
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cobalt-activated
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medicine
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diagnostics
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analysis
- 3.5.1.14
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n-acetylated
- synthesis
- n-acetyl-l-methionine
-
mercapturic
- acylases
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cobalt-activated
- medicine
- diagnostics
- analysis
Reaction
Synonyms
AA1, AA3, AAIII, ACY 1a, ACY 1b, ACY-1, ACY-1A, Acy1, acylase, acylase 1, acylase B, acylase I, alpha-N-acylaminoacid hydrolase, amido acid deacylase, aminoacylase, aminoacylase 1, aminoacylase 1a, aminoacylase 3, aminoacylase I, aminoacylase-1, aminoacylase-1A, aminoacylase-1B, benzamidase, D-AAse, D-Aminoacylase, dehydropeptidase II, DR_ACY, fatty acid-amino acid conjugate hydrolyzing aminoacylase 1, fMDF, fungus aminoacylase-1, hAcy1, hippurase, hippuricase, histozyme, HKA, hog intestinal acylase I, hog kidney aminoacylase, hog kidney aminoacylase I, intestine acylase I, L-ACY-1, L-amido-acid acylase, L-aminoacylase, LAA, lepidopteran aminoacylase 1, long acyl amidoacylase, M20, More, N-Acyl-D-aspartate amidohydrolase, N-acyl-L-amino acid amidohydrolase, N-acyl-L-amino acid hydrolase, N-acyl-L-amino-acid amidohydrolase, N-acyl-L-aminoacylase 1, N-acylase IA, N-formylmethionine deformylase, Nalpha-acyl-L-amino acid amidohydrolase, pAcy1, PhoACY, pig kidney aminoacylase, porcine kidney aminoacylase, short acyl amidoacylase, sphingosine kinase 1-interacting protein
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Turnover Number on EC 3.5.1.14 - N-acyl-aliphatic-L-amino acid amidohydrolase
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
45 - 150
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
3
2-acetylamino-3-((3S,4S)-2-hydroxycyclooctylsulfanyl)-propionic acid
analysis by HPLC, pH 7.6, 37°C
19.5 - 80
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
0.1
N-acetyl-L-aspartate
-
E167R mutant protein, pH not specified in the publication, temperature not specified in the publication
0.6
N-acetyl-L-tyrosine
-
E167R mutant protein, pH not specified in the publication, temperature not specified in the publication
177
N-formyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
200
N-formyl-Lmethionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
additional information
additional information
-
kinetics of mutants N263D, R276N, and R276Q are not measurable
-
45
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
analysis by HPLC, pH 7.6, 37°C
50
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
150
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
26
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
30
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
analysis by HPLC, pH 7.6, 37°C
120
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
19.5
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
analysis by HPLC, pH 7.6, 37°C
21
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
80
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
0.0015
N-2,2-dimethylpropionyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
0.0015
N-2,2-dimethylpropionyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
18
N-acetyl-L-Cys
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
63.4
N-acetyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1-T347S-mutant
120
N-acetyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1-T347S-mutant
156
N-acetyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1
207
N-acetyl-L-glutamate
-
kcat for the hydrolysis of N-acetyl-L-glutamate substrates by pAcy1
30
N-acetyl-L-Met
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
1.2
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347F-mutant
4.49
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant
12.1
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant
16.6
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372G-mutant
38.3
N-acetyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
46
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347G-mutant
64.6
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant
66.7
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
93.7
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant
134
N-acetyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
0.0208
N-acetyl-L-phenylalanine
pH 7.5, 85°C, mutant enzyme C102S
2
N-acetyl-L-Val
analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C
0.0101
N-benzoyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1
0.115
N-benzoyl-L-glutamate
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kcat for the hydrolysis of N-acetyl-L-glutamate substrates by pAcy1
0.158
N-benzoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
1.3
N-benzoyl-L-methionine
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kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
20.1
N-benzoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1-T347S-mutant
3620
N-benzoyl-L-phenylalanine
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S100T/M106K mutant protein, pH 8.0, 55°C
107
N-butanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
139
N-butanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.00623
N-cyclohexanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.129
N-cyclohexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant
0.145
N-cyclohexanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
11.2
N-cyclopentanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
13.3
N-cyclopentanoyl-L-methionine
kcat the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.052 - 2.1
N-hexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
4.3
N-hexanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
9.51
N-hexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
16.1
N-hexanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
39.4
N-hexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant
0.529
N-isobutanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
6.08
N-isobutanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
89.8
N-isobutanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
0.105
N-isopentanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.34
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant
0.694
N-isopentanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
2.01
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant
3.3
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant
5.36
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant
6.08
N-isopentanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
16.2
N-pentanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
18.2
N-pentanoyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
25.7
N-propionyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
168
N-propionyl-L-methionine
-
kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1
