3.5.1.14: N-acyl-aliphatic-L-amino acid amidohydrolase

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For detailed information about N-acyl-aliphatic-L-amino acid amidohydrolase, go to the full flat file.

Reaction

Synonyms

AA1, AA3, AAIII, ACY 1a, ACY 1b, ACY-1, ACY-1A, Acy1, acylase, acylase 1, acylase B, acylase I, alpha-N-acylaminoacid hydrolase, amido acid deacylase, aminoacylase, aminoacylase 1, aminoacylase 1a, aminoacylase 3, aminoacylase I, aminoacylase-1, aminoacylase-1A, aminoacylase-1B, benzamidase, D-AAse, D-Aminoacylase, dehydropeptidase II, DR_ACY, fatty acid-amino acid conjugate hydrolyzing aminoacylase 1, fMDF, fungus aminoacylase-1, hAcy1, hippurase, hippuricase, histozyme, HKA, hog intestinal acylase I, hog kidney aminoacylase, hog kidney aminoacylase I, intestine acylase I, L-ACY-1, L-amido-acid acylase, L-aminoacylase, LAA, lepidopteran aminoacylase 1, long acyl amidoacylase, M20, More, N-Acyl-D-aspartate amidohydrolase, N-acyl-L-amino acid amidohydrolase, N-acyl-L-amino acid hydrolase, N-acyl-L-amino-acid amidohydrolase, N-acyl-L-aminoacylase 1, N-acylase IA, N-formylmethionine deformylase, Nalpha-acyl-L-amino acid amidohydrolase, pAcy1, PhoACY, pig kidney aminoacylase, porcine kidney aminoacylase, short acyl amidoacylase, sphingosine kinase 1-interacting protein

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.14 N-acyl-aliphatic-L-amino acid amidohydrolase

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Results	in table
5	Activating Compound
3780	AA Sequence
31	Application
1	CAS Registry Number
27	Cloned(Commentary)
4	Crystallization (Commentary)
169	Disease/ Diagnostics
3	Expression
3	General Information
4	General Stability
1	IC50 Value
155	Inhibitors
24	kcat/KM [mM/s]
4	Ki Value [mM]
200	KM Value [mM]
8	Localization
98	Metals/Ions
68	Molecular Weight [Da]
59	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
118	Organism
5	Pathway
10	PDB ID
54	pH Optimum
10	pH Range
7	pH Stability
81	Protein Variants
42	Purification (Commentary)
4	Reaction
16	Reaction Type
111	Reference
3	Renatured (Commentary)
51	Source Tissue
129	Specific Activity [micromol/min/mg]
7	Storage Stability
628	Substrates and Products (Substrate)
58	Subunits
96	Synonyms
1	Systematic Name
34	Temperature Optimum [°C]
6	Temperature Range [°C]
21	Temperature Stability [°C]
118	Turnover Number [1/s]

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RENATURED/Commentary

ORGANISM

UNIPROT

LITERATURE

60fold dilution of guanidinum hydrochloride-denatured enzyme, role of osmolytes, e.g. glycerol, DMSO, sucrose, or proline, as chemical chaperones during the refolding of aminoacylase, off-pathway aggregation occurs, osmolytes inhibits aggregation and recover enzyme activity during refolding, overview

Sus scrofa

-

667454

assisting the reactivation of guanidine hydrochloride-denatured aminoacylase by hydroxypropyl cyclodextrins in a concentration-dependent manner, most efficiently by hydroxypropyl beta-cyclodextrin, low concentrations of hydroxypropyl alpha-cyclodextrin and high concentrations of hydroxypropyl gamma-cyclodextrin promoted off-pathway aggregation, recovery of secondary and tertiary structures, overview

Sus scrofa

-

667997

denaturation of the enzyme with 6 M guanidinium hydrochloride, dilution 60fold with Tris-HCl buffer, pH 7.5, determination of the refolding intermediate of guanidine hydrochloride denatured aminoacylase, the refolded enzyme shows 58-24% of maximal activity dependent on the temperature during refolding, 58% of native activity after refolding at 4°C, overview

Sus scrofa

-

668961