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3.5.1.14: N-acyl-aliphatic-L-amino acid amidohydrolase

This is an abbreviated version!
For detailed information about N-acyl-aliphatic-L-amino acid amidohydrolase, go to the full flat file.

Word Map on EC 3.5.1.14

Reaction

an N-acetyl-L-cysteine-S-conjugate
+
H2O
=
an L-cysteine-S-conjugate
+
acetate

Synonyms

AA1, AA3, AAIII, ACY 1a, ACY 1b, ACY-1, ACY-1A, Acy1, acylase, acylase 1, acylase B, acylase I, alpha-N-acylaminoacid hydrolase, amido acid deacylase, aminoacylase, aminoacylase 1, aminoacylase 1a, aminoacylase 3, aminoacylase I, aminoacylase-1, aminoacylase-1A, aminoacylase-1B, benzamidase, D-AAse, D-Aminoacylase, dehydropeptidase II, DR_ACY, fatty acid-amino acid conjugate hydrolyzing aminoacylase 1, fMDF, fungus aminoacylase-1, hAcy1, hippurase, hippuricase, histozyme, HKA, hog intestinal acylase I, hog kidney aminoacylase, hog kidney aminoacylase I, intestine acylase I, L-ACY-1, L-amido-acid acylase, L-aminoacylase, LAA, lepidopteran aminoacylase 1, long acyl amidoacylase, M20, More, N-Acyl-D-aspartate amidohydrolase, N-acyl-L-amino acid amidohydrolase, N-acyl-L-amino acid hydrolase, N-acyl-L-amino-acid amidohydrolase, N-acyl-L-aminoacylase 1, N-acylase IA, N-formylmethionine deformylase, Nalpha-acyl-L-amino acid amidohydrolase, pAcy1, PhoACY, pig kidney aminoacylase, porcine kidney aminoacylase, short acyl amidoacylase, sphingosine kinase 1-interacting protein

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.14 N-acyl-aliphatic-L-amino acid amidohydrolase

Purification

Purification on EC 3.5.1.14 - N-acyl-aliphatic-L-amino acid amidohydrolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, octyl-Sepharose column chromatography, HiTrap chelating column chromatography, and DEAE Sephadex A-50 ion exchange chromatography
anion exchange chromatography, hydrophobic interaction chromatography
-
both short acyl aminoacylase and long acyl aminoacylase
-
by a method that includes DEAE-Sepharose column, Chelating-Sepharose column, Amicon PM 30 membrane and Sephacryl-S200 column
-
commercial preparation
from frass: ion exchange chromatography (Q-Sepharose), gel filtration, ion exchange chromatography (Mono-Q, gel filtration), recombinant protein from inclusion bodies: immobilized metal ion affinity chromatography (Co2+)
in 50 mM Tris/HCl-buffer, pH 8 and disrupted by ultrasonication
native enzyme 1300fold by ammonium sulfate fractionation, dialysis, ion exchange chromatography, hydroxyapatite chromatography, and a second step of dialysis
-
native enzyme from intestinal epithelial mucosa, 1833fold to homogeneity, by acid precipitation, two steps of anion exchange chromatography, hydrophobic interaction chromatography, gel filtration and concanavalin A afinity chromatography
-
native enzyme from kidney by acetone precipitation, ion exchange chromatography, and gel filtration
-
native enzyme from kidney by acetone precipitation, ion exchange chromatography, and gel filtration to homogeneity
-
partially
-
purified by a method that includes DEAE-Sepharose CL-6B anion exchange resin, phenyl-Sepharose hydrophobic interaction resin, Mono Q strong anion exchange column and Mono P weak anion exchange column
-
recombinant GST-tagged C-terminal fragment from an in vitro expression system by glutathione affinity chromatography
-
recombinant GST-tagged wild-type and mutant enzymes from bacteria by solubilization with Triton X-100 and 10% sarcosyl, glutathione affinity chromatography, and tag removal by thrombin
recombinant protein, expressed in Escherichia coli
-
StrepTactin Sepharose
-
Streptomyces lividans strain TK24, native enzyme 494fold from by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography
using either Ni-NTA (Qiagen), Fractogel HW-50S and HiTrap Q (Pharmacia Biotech) columns or an NiNTA (Quiagen) column only
-