from frass: ion exchange chromatography (Q-Sepharose), gel filtration, ion exchange chromatography (Mono-Q, gel filtration), recombinant protein from inclusion bodies: immobilized metal ion affinity chromatography (Co2+)
native enzyme 1300fold by ammonium sulfate fractionation, dialysis, ion exchange chromatography, hydroxyapatite chromatography, and a second step of dialysis
native enzyme from intestinal epithelial mucosa, 1833fold to homogeneity, by acid precipitation, two steps of anion exchange chromatography, hydrophobic interaction chromatography, gel filtration and concanavalin A afinity chromatography
purified by a method that includes DEAE-Sepharose CL-6B anion exchange resin, phenyl-Sepharose hydrophobic interaction resin, Mono Q strong anion exchange column and Mono P weak anion exchange column
recombinant GST-tagged wild-type and mutant enzymes from bacteria by solubilization with Triton X-100 and 10% sarcosyl, glutathione affinity chromatography, and tag removal by thrombin