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3.5.1.14: N-acyl-aliphatic-L-amino acid amidohydrolase

This is an abbreviated version!
For detailed information about N-acyl-aliphatic-L-amino acid amidohydrolase, go to the full flat file.

Word Map on EC 3.5.1.14

Reaction

an N-acetyl-L-cysteine-S-conjugate
+
H2O
=
an L-cysteine-S-conjugate
+
acetate

Synonyms

AA1, AA3, AAIII, ACY 1a, ACY 1b, ACY-1, ACY-1A, Acy1, acylase, acylase 1, acylase B, acylase I, alpha-N-acylaminoacid hydrolase, amido acid deacylase, aminoacylase, aminoacylase 1, aminoacylase 1a, aminoacylase 3, aminoacylase I, aminoacylase-1, aminoacylase-1A, aminoacylase-1B, benzamidase, D-AAse, D-Aminoacylase, dehydropeptidase II, DR_ACY, fatty acid-amino acid conjugate hydrolyzing aminoacylase 1, fMDF, fungus aminoacylase-1, hAcy1, hippurase, hippuricase, histozyme, HKA, hog intestinal acylase I, hog kidney aminoacylase, hog kidney aminoacylase I, intestine acylase I, L-ACY-1, L-amido-acid acylase, L-aminoacylase, LAA, lepidopteran aminoacylase 1, long acyl amidoacylase, M20, More, N-Acyl-D-aspartate amidohydrolase, N-acyl-L-amino acid amidohydrolase, N-acyl-L-amino acid hydrolase, N-acyl-L-amino-acid amidohydrolase, N-acyl-L-aminoacylase 1, N-acylase IA, N-formylmethionine deformylase, Nalpha-acyl-L-amino acid amidohydrolase, pAcy1, PhoACY, pig kidney aminoacylase, porcine kidney aminoacylase, short acyl amidoacylase, sphingosine kinase 1-interacting protein

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.14 N-acyl-aliphatic-L-amino acid amidohydrolase

Cloned

Cloned on EC 3.5.1.14 - N-acyl-aliphatic-L-amino acid amidohydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ACYI, DNA and amino acid sequence determination and analysis, comparison with the porcine intestinal enzyme
ACYI, DNA and amino acid sequence determination and analysis, comparison with the rat kidney enzyme
cloning of Acy1 from a mouse kidney library, expression of the C-terminal fragment in HEK-293 cells, in vitro expression of the GST-tagged C-terminal fragment, expression as myc-tagged enzyme in Cos-7 cells, expression with murine sphingosine kinase type 1, SphK1, in a yeast two-hybrid system
-
DNA and amino acid sequence determination and analysis, sequence comparisons, subcloning in Escherichia coli strain DH5alpha, expression in Streptomyces lividans strain TK24 resulting in 42fold higher activity compared to the native wild-type Streptomyces mobaraensis strain
expressed in Escherichia coli
expressed in Escherichia coli strain BL21(DE3)
-
expressed in Streptomyces lividans strain TK24
expression in Acremonium chrysogenum IS-5, transformants with pSACY2 or pMACY2
-
expression in Baculovirus
expression in E. coli
expression in Escherichia coli
expression in HEK293T cells
expression of GST-tagged wild-type and mutant enzymes in bacteria
expression of His6-tagged AA3 in HEK-293 cells
-
expression of pAcy1 in Escherichia coli strain BL21 (DE3)
genomic DNA fragment encoding aminoacylase cloned, sequenced and expressed in Escherichia coli
-
overexpressed in Escherichia coli
-
overexpressed in Escherichia coli, modification of N-terminal residues of the recombinant enzyme to enhance expression in Escherichia coli using the vector pUCcer11. The recombinant enzyme has an insertion of four amino acids (Glu-Pro-Leu-Phe) between the N-terminal methionine and the second residue lysine of the wild type
-