3.5.1.13: aryl-acylamidase

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For detailed information about aryl-acylamidase, go to the full flat file.

Reaction

Synonyms

AAA, aryl acylamidase, aryl acylaminidase, Azl13, EC 3.5.1.13 AAA-2, EC 3.5.1.13, AAA-1, M-1 amidase, More, PamH, polyamidase, pseudocholinesterase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.13 aryl-acylamidase

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Results	in table
13	Activating Compound
531	AA Sequence
5	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	Crystallization (Commentary)
3	Disease/ Diagnostics
10	General Information
1	General Stability
3	IC50 Value
206	Inhibitors
3	kcat/KM [mM/s]
15	Ki Value [mM]
51	KM Value [mM]
13	Localization
8	Metals/Ions
14	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
92	Organism
2	PDB ID
40	pH Optimum
9	pH Range
7	pH Stability
2	pI Value
1	Posttranslational Modification
13	Protein Variants
23	Purification (Commentary)
3	Reaction
3	Reaction Type
90	Reference
54	Source Tissue
50	Specific Activity [micromol/min/mg]
7	Storage Stability
205	Substrates and Products (Substrate)
14	Subunits
40	Synonyms
1	Systematic Name
33	Temperature Optimum [°C]
3	Temperature Range [°C]
25	Temperature Stability [°C]
19	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.5.1.13 - aryl-acylamidase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-15°C, stable for 3 months

Lysinibacillus sphaericus

-

208988

-15°C, stable for several months

Glycine max

-

208986

-18°C, 1 week, 18% loss of activity

Nocardia farcinica

-

696876

25°C, 1 week, 5% loss of activity

Nocardia farcinica

-

696876

4°C or 20°C, stable for 420 min

Pseudomonas fluorescens

-

208973

4°C, storage after freeze-drying in buffer containing 5% lactose, about 12% loss of activity

Pseudomonas fluorescens

-

208973

rapid loss of activity during purification and storage in Tris-HCl (10 mM, pH 8.0) buffer at 4°C or 20°C, with the addition of 10% glycerol and 50 mM NaCl prior to freezing activity is maintained for up to 3 months with only marginal losses

Paracoccus sp. M1-1

F6N111

718672