3.5.1.13: aryl-acylamidase

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For detailed information about aryl-acylamidase, go to the full flat file.

Reaction

Synonyms

AAA, aryl acylamidase, aryl acylaminidase, Azl13, EC 3.5.1.13 AAA-2, EC 3.5.1.13, AAA-1, M-1 amidase, More, PamH, polyamidase, pseudocholinesterase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.13 aryl-acylamidase

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Results	in table
13	Activating Compound
531	AA Sequence
5	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	Crystallization (Commentary)
3	Disease/ Diagnostics
10	General Information
1	General Stability
3	IC50 Value
206	Inhibitors
3	kcat/KM [mM/s]
15	Ki Value [mM]
51	KM Value [mM]
13	Localization
8	Metals/Ions
14	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
92	Organism
2	PDB ID
40	pH Optimum
9	pH Range
7	pH Stability
2	pI Value
1	Posttranslational Modification
13	Protein Variants
23	Purification (Commentary)
3	Reaction
3	Reaction Type
90	Reference
54	Source Tissue
50	Specific Activity [micromol/min/mg]
7	Storage Stability
205	Substrates and Products (Substrate)
14	Subunits
40	Synonyms
1	Systematic Name
33	Temperature Optimum [°C]
3	Temperature Range [°C]
25	Temperature Stability [°C]
19	Turnover Number [1/s]

Purification

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Purification on EC 3.5.1.13 - aryl-acylamidase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

11 entries

ammonium sulfate fractionation, ion exchange chromatography (DEAE-Sephadex, DEAE-Cellulose, gel filtration)

Paracoccus sp. M1-1

F6N111

718672

immobilized metal ion affinity chromatography (Ni2+)

Nocardia farcinica

-

720273

native enzyme from brain by ammonium sulfate fractionation and procainamide affinity chromatography

Gallus gallus

-

711318

native enzymes by immunoaffinity chromatography

Gallus gallus

-

670800

partial

2 entries

recombinant His6-tagged PamH 5.8fold from Escherichia coli strain BL21 (DE3) by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration

Paracoccus sp. M1-1

F6N111

718672

recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Streptomyces sp.

A0A088BHP3

733190

recombinant His6-tagged wild-type enzyme 5.82fold from Escherichia coli strain BL21 (DE3) by ammonium sulfate fractionation, dialysis, anion exchange chromatography, gel filtration, and ultrafiltration

Paracoccus sp. M1-1

F6N111

718672

serotonin-sensitive enzyme

Platyrrhini

-

208979

-

Homo sapiens

-

208978, 208997, 697867

-

Bos taurus

-

697902

-

Ovis aries

-

208978

-

Glycine max

-

208986

-

Lysinibacillus sphaericus

-

208988

-

Pseudomonas fluorescens

-

208973

-

Rhodococcus erythropolis

-

208995

-

Electrophorus electricus

-

208978

-

Tulipa gesneriana

-

208993

-

Nocardia farcinica

-

696876

-

bacterium CSBL00001

C3UWD1

752657

partial

Homo sapiens

-

208977

partial

Taraxacum officinale

-

208985