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Literature summary for 3.5.1.13 extracted from

  • Hoagland, R.E.; Graf, G.
    The purification and properties of an amidohydrolase from soybean (1974), Can. J. Biochem., 52, 903-910.
    View publication on PubMed
Search for more literature for 3.5.1.13

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.5 mM AgCl, complete inhibition Glycine max
catechol 0.5 mM, 8.5% inhibition Glycine max
Cu2+ 0.5 mM CuSO4, 65.5% loss of activity Glycine max
HgCl2
-
 Glycine max
NEM
-
 Glycine max
Ni2+ 0.2 mM NiCl2, 50.2% inhibition Glycine max
p-benzoquinone
-
 Glycine max
PCMB
-
 Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0578
-
 Nalpha-benzoyl-DL-Arg 2-naphthylamide
-
 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
root roots of seedlings Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.81
-
-
 Glycine max

Storage Stability

Storage Stability Organism
-15°C, stable for several months Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-2-naphthylamide + H2O
-
 Glycine max Gly 2-naphthylamine
-
 ?
Gly-p-nitroanilide + H2O
-
 Glycine max Gly + p-nitroaniline
-
 ?
L-Ala beta-naphthylamide + H2O
-
 Glycine max Ala + beta-naphthylamine
-
 ?
L-Arg beta-naphthylamide + H2O
-
 Glycine max Arg + beta-naphthylamine
-
 ?
L-Leu beta-naphthylamide + H2O
-
 Glycine max Leu + beta-naphthylamine
-
 ?
L-Leu p-nitroanilide + H2O
-
 Glycine max Leu + p-nitroaniline
-
 ?
L-Leu-L-Ala + H2O
-
 Glycine max Leu + Ala
-
 ?
L-Leu-L-Phe + H2O
-
 Glycine max Leu + Phe
-
 ?
L-Leu-L-Tyr + H2O
-
 Glycine max Leu + Tyr
-
 ?
L-Leu-L-Val + H2O
-
 Glycine max Leu + Val
-
 ?
L-Lys beta-naphthylamide + H2O
-
 Glycine max Lys + beta-naphthylamine
-
 ?
L-Met beta-naphthylamide + H2O
-
 Glycine max Met + beta-naphthylamine
-
 ?
Nalpha-benzoyl-DL-Arg beta-naphthylamide + H2O
-
 Glycine max Nalpha-benzoyl-DL-Arg + beta-naphthylamine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 hydrolysis of Nalpha-benzoyl-DL-Arg p-nitroanilide Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 5 min, stable up to Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Glycine max