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Literature summary for 3.5.1.13 extracted from
- Purification and properties of an aryl acylamidase of Bacillus sphaericus, catalyzing the hydrolysis of various phenylamide herbicides and fungicides (1973), Appl. Microbiol., 26, 709-718.
General Stability
| General Stability | Organism |
|---|---|
| urea, 6 M, irreversible inactivation | Lysinibacillus sphaericus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,4-dichloroaniline | - |
Lysinibacillus sphaericus |  |
| Ag+ | 0.1 mM, 6% inhibition | Lysinibacillus sphaericus | |
| Cu2+ | 0.1 mM, 9.5% inhibition | Lysinibacillus sphaericus | |
| HgCl2 | 0.1 mM, 98% inhibition | Lysinibacillus sphaericus | |
| iodoacetamide | - |
Lysinibacillus sphaericus | |
| PCMB | - |
Lysinibacillus sphaericus | |
| puromycin | competitive inhibition of hydrolysis L-Ala-4-nitroanilide | Lysinibacillus sphaericus | |
| Sodium arsenate | - |
Lysinibacillus sphaericus | |
| Urea | 6 M, irreversible inactivation | Lysinibacillus sphaericus | |
| Zn2+ | 0.1 mM, 51% inhibition | Lysinibacillus sphaericus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea | - |
Lysinibacillus sphaericus | |
| 0.03 | - |
2,5-dimethylfuran-3-carboxanilide | - |
Lysinibacillus sphaericus | |
| 0.09 | - |
L-Ala 4-nitroanilide | - |
Lysinibacillus sphaericus | |
| 0.2 | - |
L-Leu 4-nitroanilide | - |
Lysinibacillus sphaericus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Lysinibacillus sphaericus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Lysinibacillus sphaericus | inducible by various phenylamides of the acylanilide, phenylcarbamate, and methoxysubstituted phenylurea type | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysinibacillus sphaericus | - |
ATCC 12123 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lysinibacillus sphaericus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, stable for 3 months | Lysinibacillus sphaericus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,5-dimethylfuran-3-carboxanilide + H2O | - |
Lysinibacillus sphaericus | ? | - |
? | |
| 3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea + H2O | i.e. linuron | Lysinibacillus sphaericus | 3,4-dichloroaniline + ? | - |
? | |
| L-Ala-4-nitroanilide + H2O | - |
Lysinibacillus sphaericus | Ala + p-nitroaniline | - |
? | |
| L-Leu p-nitroanilide + H2O | - |
Lysinibacillus sphaericus | Leu + p-nitroaniline | - |
? | |
| additional information | inducible by various phenylamides of the acylanilide, phenylcarbamate, and methoxysubstituted phenylurea type | Lysinibacillus sphaericus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
9.5 min, 50% loss of activity | Lysinibacillus sphaericus |
| 45 | - |
2 min, 50% loss of activity | Lysinibacillus sphaericus |
| 50 | - |
0.5 min, 50% loss of activity | Lysinibacillus sphaericus |
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